Ferritin From Striped Stem Borer (Chilo suppressalis) Oral Secretion Acts as an Effector Helping to Maintain Iron Homoeostasis and Impair Defenses in Rice

IF 6 1区 生物学 Q1 PLANT SCIENCES
Shan Yu, Shuai Li, Jing Li, Chen-Yang Wang, Lei Yang, Jing Li, Yue Meng, Xin-Yang Tan, Mao-Feng Jing, Guang-Hua Luo, Ji-Chao Fang, Rui Ji
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Abstract

The striped stem borer (Chilo suppressalis, SSB) is a highly destructive insect pest in rice (Oryza sativa). SSB oral secretions (OSs) can induce plant defense responses in rice. However, the specific effectors in SSB OSs that mediate these interactions with rice remain poorly understood. In this study, hallmarks of ferroptosis-like plant defense response, such as the reprogramming of ferroptosis-related genes, reduced glutathione levels, accumulation of ferric ion, and enhanced lipid peroxidation by reactive oxygen species (ROS), were detected in rice subjected to SSB infestation and SSB OSs treatment. Furthermore, we identified and characterized a protein from SSB OSs, the ferritin CsFer1, which plays a critical role in the regulation of plant iron homoeostasis. CsFer1 was shown to possess Fe2+ binding capacity and ferroxidase activity. Through recombinant CsFer1 protein treatment, overexpression of CsFer1 in rice and SSB larvae with silencing CsFer1 feeding in rice, we found that CsFer1 helped maintain iron homoeostasis under SSB infestation, suppressing H2O2 and JA accumulation, ultimately compromising rice resistance to herbivorous pests. Moreover, such a phenomenon about the regulation of iron homoeostasis and suppression of insect resistance was observed in the CsFer1 overexpressed tobacco. Collectively, these findings suggest that CsFer1 functions as an effector involved in the regulation of iron homoeostasis- and lipid peroxidation-related plant defense during plant-insect interaction.

条纹茎螟(Chilo suppressalis)口腔分泌的铁蛋白有助于维持水稻铁平衡和削弱防御。
条纹螟虫(Chilo suppressalis, SSB)是水稻的一种极具破坏性的害虫。SSB口腔分泌物可诱导水稻植株防御反应。然而,SSB os中介导这些与水稻相互作用的特定效应物仍然知之甚少。在本研究中,研究人员检测到在SSB侵染和SSB OSs处理的水稻中,出现了类似植物防御反应的特征,如与铁中毒相关基因的重编程、谷胱甘肽水平的降低、铁离子的积累以及活性氧(ROS)对脂质过氧化的增强。此外,我们从SSB os中鉴定并鉴定了铁蛋白CsFer1,该蛋白在植物铁稳态调节中起关键作用。CsFer1具有Fe2+结合能力和铁氧化酶活性。通过重组CsFer1蛋白处理,在水稻和SSB幼虫中过表达CsFer1并沉默CsFer1摄食水稻,我们发现CsFer1有助于维持SSB侵染下的铁稳态,抑制H2O2和JA积累,最终影响水稻对草食性害虫的抗性。此外,在CsFer1过表达的烟草中也观察到这种调节铁稳态和抑制昆虫抗性的现象。综上所述,这些发现表明CsFer1在植物-昆虫相互作用中作为一种效应因子参与调节铁稳态和脂质过氧化相关的植物防御。
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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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