3D Printed Titanium Scaffolds with Bi-Directional Gradient QK-Functionalized Surface

IF 27.4 1区 材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Xiaoyun Sun, Ru Zhong, Congcong Wu, Silin Ye, Haipeng Yuan, Zhou Fang, Junjian Chen, Delin Cheng, Lijing Hao, Lei Chu, Lin Wang
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Abstract

3D printed titanium scaffold has promising applications in orthopedics. However, the bioinert titanium presents challenges for promoting vascularization and tissue growth within the porous scaffold for stable osteointegration. In this study, a modular porous titanium scaffold is created using 3D printing and a gradient-surface strategy to immobilize QK peptide on the surface with a bi-directional gradient distribution. This design featured high peptide density in the interior and low peptide density on both ends, aiming to induce cell migration from ends to interior and subsequently enhance vascularization and osteointegration within the scaffold. In vitro results showed that besides the inherent bioactivity, the gradient distribution of QK positively correlated with endothelial cell migration and promoted angiogenesis. In vivo assay was performed by a segmental bone defect model in rabbit and a spine repair model in sheep. Various staining and Micro-CT results demonstrated that compared to that with uniformly QK-functionalized surface, the scaffold with bi-directional gradient QK-functionalized surface (Ti-G) significantly encouraged new tissue growth toward the interior of the scaffold, subsequently facilitated angiogenesis and osteointegration. This study provides an effective strategy for enhancing the bioactivity of peptide-functionalized scaffolds through the concept of bi-directional gradients, and holds potential for various 3D printed scaffolds.

Abstract Image

Abstract Image

具有双向梯度qk功能化表面的3D打印钛支架
3D打印钛支架在骨科中有很好的应用前景。然而,生物惰性钛在促进多孔支架内血管化和组织生长以实现稳定的骨整合方面存在挑战。在本研究中,采用3D打印和梯度表面策略,以双向梯度分布的方式将QK肽固定在表面上,构建了模块化多孔钛支架。该设计的特点是内部高肽密度,两端低肽密度,旨在诱导细胞从末端向内部迁移,从而增强支架内的血管化和骨整合。体外实验结果显示,除了固有的生物活性外,QK的梯度分布与内皮细胞迁移和促进血管生成呈正相关。采用兔节段性骨缺损模型和羊脊柱修复模型进行体内实验。各种染色和Micro-CT结果表明,与具有均匀qk功能化表面的支架相比,具有双向梯度qk功能化表面(Ti-G)的支架显著促进新组织向支架内部生长,从而促进血管生成和骨整合。本研究通过双向梯度的概念为增强肽功能化支架的生物活性提供了一种有效的策略,并为各种3D打印支架提供了潜力。
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来源期刊
Advanced Materials
Advanced Materials 工程技术-材料科学:综合
CiteScore
43.00
自引率
4.10%
发文量
2182
审稿时长
2 months
期刊介绍: Advanced Materials, one of the world's most prestigious journals and the foundation of the Advanced portfolio, is the home of choice for best-in-class materials science for more than 30 years. Following this fast-growing and interdisciplinary field, we are considering and publishing the most important discoveries on any and all materials from materials scientists, chemists, physicists, engineers as well as health and life scientists and bringing you the latest results and trends in modern materials-related research every week.
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