Unignorable environmental risks: Insight into differential responses between biofilm and plastisphere in sulfur autotrophic denitrification system upon exposure to quaternary ammonium compounds

Abstract

Concerns of quaternary ammonium compounds (QACs) and microplastics (MPs) as emerging containments accumulating in wastewater treatment plants (WWTPs) have attracted much attention. Plastisphere with distinctive microbial communities might also be the repository for pathogens and resistance genes (RGs). Thus, the effects of three representative QACs with different concentrations on biofilm and plastisphere were studied in sulfur autotrophic denitrification (SAD) system. Over 100 days, 1-5 mg/L QACs exerted few impacts on system stability, whereas 15 mg/L QACs seriously lowered the microbial activity and the inhibitory effects ranked: benzylalkyldimethylethyl ammonium compound > dialkyldimethyl ammonium compound > alkyltrimethyl ammonium compound. Dosing of QACs in SAD system not only altered the microbial community structure and assembly, but also induced higher levels of intracellular RGs and extracellular RGs in plastisphere than in biofilm. Although the free RGs abundances in water slightly lowered, they might also pose great ecological risks. Pathogens identified as the potential hosts of RGs were more prone to colocalizing in plastisphere. Mobile genetic elements directly contributed to the three-fraction RGs transmission in SAD system. This study offered new insights into the differential responses of biofilm and plastisphere under QAC stress and guided for the disinfectants and MPs pollution containment in WWTPs.