Biochar reduces plasmid-mediated antibiotic resistance gene transfer in earthworm ecological filters for rural sewage treatment

IF 12.2 1区 环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL
Meng Zhao, Kui Huang, Feifei Wen, Hui Xia, Bingyu Song
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Abstract

The spread of antibiotic resistance genes (ARGs) in rural wastewater threatens both ecological environment and human health. Earthworm ecological filters (EEFs) represent a green technology for rural sewage treatment. However, their effectiveness in removing ARGs remains a significant challenge. This study aims to investigate the role and underlying mechanisms of biochar addition in enhancing ARGs removal in rural sewage using EEFs. To achieve this, the fate of chromosome- and plasmid-carried ARGs was quantified in constructed EEFs, both with and without biochar addition. The results showed that the biochar could effectively remove ARGs from rural sewage, with a better removal efficiency for plasmid-carried ARGs. The absolute abundance of plasmid-carried ARGs in the effluent was reduced by 0.4 to 11 times compared to chromosomal ones, showing removal stability improved by 13.11% to 74.51%. Additionally, the functional microbial community attached on the high porosity of biochar surface promoted ARGs retention, increasing diffusion limitation in microbial assembly mechanisms by 4.61-29.44%, which played a key role in plasmid-mediated horizontal gene transfer (HGT). Partial least squares structural equation modeling (PLS-SEM) revealed that biochar-mediated environmental changes and the HGT of mobile genetic elements (MGEs) were critical factors in reducing plasmid-carried ARGs in EEFs.

Abstract Image

生物炭减少了质粒介导的抗生素抗性基因在蚯蚓生态过滤器中的转移,用于农村污水处理
农村废水中抗生素耐药基因(ARGs)的传播对生态环境和人类健康构成威胁。蚯蚓生态过滤器(EEFs)是一种绿色的农村污水处理技术。然而,它们在移除arg方面的有效性仍然是一大挑战。本研究旨在探讨添加生物炭对EEFs去除农村污水中ARGs的作用及其机制。为了实现这一点,在构建的EEFs中,在添加和不添加生物炭的情况下,对携带染色体和质粒的ARGs的命运进行了量化。结果表明,生物炭能有效去除农村污水中的ARGs,对质粒携带的ARGs去除效果较好。出水中携带质粒的ARGs的绝对丰度比携带染色体的ARGs的绝对丰度降低了0.4 ~ 11倍,去除稳定性提高了13.11% ~ 74.51%。此外,附着在高孔隙度生物炭表面的功能微生物群落促进了ARGs的保留,使微生物组装机制的扩散限制提高了4.61 ~ 29.44%,这在质粒介导的水平基因转移(HGT)中发挥了关键作用。偏最小二乘结构方程模型(PLS-SEM)显示,生物炭介导的环境变化和移动遗传元件(MGEs)的HGT是EEFs中质粒携带的ARGs减少的关键因素。
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来源期刊
Journal of Hazardous Materials
Journal of Hazardous Materials 工程技术-工程:环境
CiteScore
25.40
自引率
5.90%
发文量
3059
审稿时长
58 days
期刊介绍: The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.
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