Sucrose non-fermenting 1-related protein kinase 2–14 participating in lipid elevating efficacy and biodiesel upgrade by Coccomyxa subllipsoidea

IF 13.3 1区工程技术 Q1 ENGINEERING, CHEMICAL

Chemical Engineering Journal Pub Date : 2025-01-15 DOI:10.1016/j.cej.2025.159607

Yuanyuan Luo, Sisi Zhao, Zhixuan Fan, Yuqin Li, Zongfan Peng, Yulong Zhang, Siran Feng, Jinhua Mou, Zhenyao Wang, Carol Sze Ki Lin, Xuan Li

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引用次数: 0

Abstract

Microalgae-based biodiesel offered a promising solution to mitigate environmental and energy crises, yet improving lipid productivity and upgrading biodiesel quality in microalgae remained a challenge. Abscisic acid (ABA) supplementation to Coccomyxa subllipsoidea resulted in lipid accumulation, and most sucrose non-fermenting 1-related protein kinase 2 (SnRK2) family genes especially SnRK2-14 containing cis-acting element for ABA responsiveness in region of promoter showed significant up-regulation and strongly positive correlated with lipid productivity. Therefore, this study was the first to explore the lipid elevating efficacy and biodiesel upgrade by SnRK2-14 overexpression in C. subllipsoidea. The lipid yield of SnRK2-14 overexpression C. subllipsoidea strain (SnRK2-14-OCS) boosted to 2.18 g/L, representing a 2.63-fold improvement over wild type (WT). Further, SnRK2-14 overexpression up-regulated lipid-related genes, rewired intermediates/energy derived from protein and carbohydrate degradation, and inhibited lipid oxidation by strengthening antioxidant capacity to collectively achieve lipid overaccumulation in SnRK2-14-OCS. Under fed-batch fermentation mode, the lipid yield further increased 7.92-fold in SnRK2-14-OCS compared to WT and also modulated fatty acid profiles to upgrade biodiesel quality to meet the established quality criteria. These findings revealed the involvement of SnRK2-14 in lipid biosynthesis in microalgae and highlighted the potential of manipulating SnRK2-14 for improved lipid and biodiesel production.

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来源期刊

Chemical Engineering Journal 工程技术-工程：化工

CiteScore

21.70

自引率

9.30%

发文量

6781

审稿时长

2.4 months

期刊介绍： The Chemical Engineering Journal is an international research journal that invites contributions of original and novel fundamental research. It aims to provide an international platform for presenting original fundamental research, interpretative reviews, and discussions on new developments in chemical engineering. The journal welcomes papers that describe novel theory and its practical application, as well as those that demonstrate the transfer of techniques from other disciplines. It also welcomes reports on carefully conducted experimental work that is soundly interpreted. The main focus of the journal is on original and rigorous research results that have broad significance. The Catalysis section within the Chemical Engineering Journal focuses specifically on Experimental and Theoretical studies in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. These studies have industrial impact on various sectors such as chemicals, energy, materials, foods, healthcare, and environmental protection.