{"title":"Novel sterilization method of Bacillus atrophaeus and Geobacillus stearothermophilus spores by low concentration chlorine dioxide gas.","authors":"Koushirou Sogawa, Hideaki Tagishi, Hideaki Kato, Takashi Shibata, Takanori Miura","doi":"10.4265/jmc.29.4_153","DOIUrl":null,"url":null,"abstract":"<p><p>Chlorine dioxide (ClO<sub>2</sub>) is a powerful disinfectant widely regarded as a safe and effective hygienic agent in pharmaceutical plants and other manufacturing facilities that require sterility. However, the efficacy of low concentrations of ClO<sub>2</sub> gas on sterilizing spore-forming bacteria remains uncertain. In this study, we investigated the optimal conditions for disinfection of spore-forming bacteria with low concentrations of ClO<sub>2</sub> gas using biological indicators. The results showed that 0.05 ppmv (0.13 mg/m<sup>3</sup>) ClO<sub>2</sub> gas at 37±1℃, 86±2%RH sterilized Bacillus atrophaeus in 14 d and Geobacillus stearothermophilus in 28 d (>6 log<sub>10</sub> reductions）. Based on these results, we propose that low concentrations of ClO<sub>2</sub> gas are useful for controlling contamination by spore-forming bacteria in aseptic facilities.</p>","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"29 4","pages":"153-156"},"PeriodicalIF":0.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of microorganism control","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4265/jmc.29.4_153","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Chlorine dioxide (ClO2) is a powerful disinfectant widely regarded as a safe and effective hygienic agent in pharmaceutical plants and other manufacturing facilities that require sterility. However, the efficacy of low concentrations of ClO2 gas on sterilizing spore-forming bacteria remains uncertain. In this study, we investigated the optimal conditions for disinfection of spore-forming bacteria with low concentrations of ClO2 gas using biological indicators. The results showed that 0.05 ppmv (0.13 mg/m3) ClO2 gas at 37±1℃, 86±2%RH sterilized Bacillus atrophaeus in 14 d and Geobacillus stearothermophilus in 28 d (>6 log10 reductions）. Based on these results, we propose that low concentrations of ClO2 gas are useful for controlling contamination by spore-forming bacteria in aseptic facilities.
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