[Regulation of Bifidobacterium-short chain fatty acid metabolism and improvement of intestinal toxicity of vinegar-processed Euphorbiae Pekinensis Radix].

Q3 Pharmacology, Toxicology and Pharmaceutics
Ling-Jun Ye, Xiao-Fen Xu, Sai-Ya Chen, Huan Zhang, Yi-Xuan Gan, Tao Meng, Rui Ding, Jing Li, Gang Cao, Kui-Long Wang
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引用次数: 0

Abstract

To explore the mechanism by which vinegar-processed Euphorbiae Pekinensis Radix regulates gut microbiota and reduces intestinal toxicity, this study aimed to identify key microbial communities related to vinegar-induced detoxification and verify their functions. Using a derivatization method, the study measured the content of short-chain fatty acids(SCFAs) in feces before and after vinegar-processing of Euphorbiae Pekinensis Radix. Combined with the results of previous gut microbiota sequencing, correlation analysis was used to identify key microbial communities related to SCFAs content. Through single-bacterium transplantation experiments, the role of key microbial communities in regulating SCFAs metabolism and alleviating the intestinal toxicity of Euphorbiae Pekinensis Radix was clarified. Fecal extracts were then added to a co-culture system of Caco-2 and RAW264.7 cells, and toxicity differences were evaluated using intestinal tight junction proteins and inflammatory factors as indicators. Additionally, the application of a SCFAs receptor blocker helped confirm the role of SCFAs in reducing intestinal toxicity during vinegar-processing of Euphorbiae Pekinensis Radix. The results of this study indicated that vinegar-processing of Euphorbiae Pekinensis Radix improved the decline in SCFAs content caused by the raw material. Correlation analysis revealed that Bifidobacterium was positively correlated with the levels of acetic acid, propionic acid, isobutyric acid, n-butyric acid, isovaleric acid, and n-valeric acid. RESULTS:: from single-bacterium transplantation experiments demonstrated that Bifidobacterium could mitigate the reduction in SCFAs content induced by raw Euphorbiae Pekinensis Radix, enhance the expression of tight junction proteins, and reduce intestinal inflammation. Similarly, cell experiment results confirmed that fecal extracts from Bifidobacterium-transplanted mice alleviated inflammation and increased the expression of tight junction proteins in intestinal epithelial cells. The use of the free fatty acid receptor-2 inhibitor GLPG0974 verified that this improvement effect was related to the SCFAs pathway. This study demonstrates that Bifidobacterium is the key microbial community responsible for reducing intestinal toxicity in vinegar-processed Euphorbiae Pekinensis Radix. Vinegar-processing increases the abundance of Bifidobacterium, elevates the intestinal SCFAs content, inhibits intestinal inflammation, and enhances the expression of tight junction proteins, thereby improving the intestinal toxicity of Euphorbiae Pekinensis Radix.

[醋制北京大戟双歧杆菌-短链脂肪酸代谢调控及肠道毒性改善]。
为探索醋处理的北京大戟调节肠道菌群和降低肠道毒性的机制,本研究旨在鉴定与醋解毒相关的关键微生物群落并验证其功能。采用衍生化法测定了北京大戟炮制前后粪便中短链脂肪酸(SCFAs)的含量。结合之前的肠道菌群测序结果,使用相关性分析确定与SCFAs含量相关的关键微生物群落。通过单菌移植实验,明确了关键微生物群落在调节北京大戟SCFAs代谢和减轻其肠道毒性中的作用。然后将粪便提取物加入Caco-2和RAW264.7细胞共培养体系中,以肠道紧密连接蛋白和炎症因子为指标评估毒性差异。此外,SCFAs受体阻断剂的应用有助于证实SCFAs在减少北京大戟醋加工过程中的肠道毒性中的作用。本研究结果表明,对北京大戟进行醋处理可以改善原料中scfa含量的下降。相关分析表明,双歧杆菌与乙酸、丙酸、异丁酸、正丁酸、异戊酸、正戊酸水平呈正相关。结果:单菌移植实验表明,双歧杆菌可以减轻生北京参诱导的SCFAs含量降低,增强紧密连接蛋白的表达,减轻肠道炎症。同样,细胞实验结果证实,双歧杆菌移植小鼠粪便提取物减轻了炎症,增加了肠上皮细胞紧密连接蛋白的表达。使用游离脂肪酸受体-2抑制剂GLPG0974验证了这种改善效果与SCFAs途径有关。本研究表明双歧杆菌是降低醋制北京大戟中肠道毒性的关键微生物群落。醋处理增加了双歧杆菌的丰度,提高了肠道SCFAs含量,抑制了肠道炎症,增强了紧密连接蛋白的表达,从而改善了北京大麻黄的肠道毒性。
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来源期刊
Zhongguo Zhongyao Zazhi
Zhongguo Zhongyao Zazhi Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
1.50
自引率
0.00%
发文量
581
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