Ambivalent partnership of the Drosophila posterior class Hox protein Abdominal-B with Extradenticle and Homothorax.

IF 4 2区 生物学 Q1 GENETICS & HEREDITY
PLoS Genetics Pub Date : 2025-01-13 eCollection Date: 2025-01-01 DOI:10.1371/journal.pgen.1011355
Jesús R Curt, Paloma Martín, David Foronda, Bruno Hudry, Ramakrishnan Kannan, Srividya Shetty, Samir Merabet, Andrew J Saurin, Yacine Graba, Ernesto Sánchez-Herrero
{"title":"Ambivalent partnership of the Drosophila posterior class Hox protein Abdominal-B with Extradenticle and Homothorax.","authors":"Jesús R Curt, Paloma Martín, David Foronda, Bruno Hudry, Ramakrishnan Kannan, Srividya Shetty, Samir Merabet, Andrew J Saurin, Yacine Graba, Ernesto Sánchez-Herrero","doi":"10.1371/journal.pgen.1011355","DOIUrl":null,"url":null,"abstract":"<p><p>Hox proteins, a sub-group of the homeodomain (HD) transcription factor family, provide positional information for axial patterning in development and evolution. Hox protein functional specificity is reached, at least in part, through interactions with Pbc (Extradenticle (Exd) in Drosophila) and Meis/Prep (Homothorax (Hth) in Drosophila) proteins. Most of our current knowledge of Hox protein specificity stems from the study of anterior and central Hox proteins, identifying the molecular and structural bases for Hox/Pbc/Meis-Prep cooperative action. Posterior Hox class proteins, Abdominal-B (Abd-B) in Drosophila and Hox9-13 in vertebrates, have been comparatively less studied. They strongly diverge from anterior and central class Hox proteins, with a low degree of HD sequence conservation and the absence of a core canonical Pbc interaction motif. Here we explore how Abd-B function interface with that of Exd/Hth using several developmental contexts, studying mutual expression control, functional dependency and intrinsic protein requirements. Results identify cross-regulatory interactions setting relative expression and activity levels required for proper development. They also reveal organ-specific requirement and a binary functional interplay with Exd and Hth, either antagonistic, as previously reported, or synergistic. This highlights context specific use of Exd/Hth, and a similar context specific use of Abd-B intrinsic protein requirements.</p>","PeriodicalId":49007,"journal":{"name":"PLoS Genetics","volume":"21 1","pages":"e1011355"},"PeriodicalIF":4.0000,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759358/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"PLoS Genetics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1371/journal.pgen.1011355","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0

Abstract

Hox proteins, a sub-group of the homeodomain (HD) transcription factor family, provide positional information for axial patterning in development and evolution. Hox protein functional specificity is reached, at least in part, through interactions with Pbc (Extradenticle (Exd) in Drosophila) and Meis/Prep (Homothorax (Hth) in Drosophila) proteins. Most of our current knowledge of Hox protein specificity stems from the study of anterior and central Hox proteins, identifying the molecular and structural bases for Hox/Pbc/Meis-Prep cooperative action. Posterior Hox class proteins, Abdominal-B (Abd-B) in Drosophila and Hox9-13 in vertebrates, have been comparatively less studied. They strongly diverge from anterior and central class Hox proteins, with a low degree of HD sequence conservation and the absence of a core canonical Pbc interaction motif. Here we explore how Abd-B function interface with that of Exd/Hth using several developmental contexts, studying mutual expression control, functional dependency and intrinsic protein requirements. Results identify cross-regulatory interactions setting relative expression and activity levels required for proper development. They also reveal organ-specific requirement and a binary functional interplay with Exd and Hth, either antagonistic, as previously reported, or synergistic. This highlights context specific use of Exd/Hth, and a similar context specific use of Abd-B intrinsic protein requirements.

果蝇后类Hox蛋白腹部- b与外胸和同胸的矛盾关系。
Hox蛋白是同源结构域(HD)转录因子家族的一个亚群,为发育和进化中的轴向模式提供位置信息。Hox蛋白的功能特异性至少部分是通过与Pbc(果蝇的extraenticle (Exd))和Meis/Prep(果蝇的Homothorax (Hth))蛋白的相互作用而实现的。我们目前对Hox蛋白特异性的大部分知识来自于对Hox蛋白前侧和中央侧的研究,确定Hox/Pbc/Meis-Prep协同作用的分子和结构基础。后Hox类蛋白,果蝇中的Abd-B和脊椎动物中的Hox9-13,研究相对较少。它们与Hox蛋白的前部和中部有很大的差异,具有低程度的HD序列保守性和缺乏核心规范的Pbc相互作用基序。在这里,我们探讨了Abd-B功能如何与Exd/Hth功能结合,研究了相互表达控制、功能依赖和内在蛋白质需求。结果确定了交叉调控相互作用,确定了适当发育所需的相对表达和活性水平。它们还揭示了Exd和Hth的器官特异性需求和二元功能相互作用,如先前报道的那样是拮抗的,或协同的。这突出了Exd/Hth的上下文特定使用,以及类似的Abd-B内在蛋白质需求的上下文特定使用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
PLoS Genetics
PLoS Genetics GENETICS & HEREDITY-
自引率
2.20%
发文量
438
期刊介绍: PLOS Genetics is run by an international Editorial Board, headed by the Editors-in-Chief, Greg Barsh (HudsonAlpha Institute of Biotechnology, and Stanford University School of Medicine) and Greg Copenhaver (The University of North Carolina at Chapel Hill). Articles published in PLOS Genetics are archived in PubMed Central and cited in PubMed.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信