Function and Potential ceRNA Identification of Circ_009773 in Neodymium Oxide Nanoparticle-Induced Lung Epithelial Mesenchymal Transition.

IF 3.9 3区 环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES
Toxics Pub Date : 2024-12-18 DOI:10.3390/toxics12120917
Lei Gao, Juan Juan, Zimeng Zheng, Lihua Huang
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引用次数: 0

Abstract

Nanoparticles of neodymium oxide (NPs-Nd2O3) can induce respiratory-related diseases, including lung tissue injury when entering the organism through the respiratory tract. However, it is currently unclear whether they can induce epithelial-mesenchymal transition (EMT) in lung tissue and the related mechanisms. In this study, we investigated the function of circ_009773 in the process of EMT induced by NPs-Nd2O3 in lung tissue from in vivo as well as in vitro experiments. The findings showed that NPs-Nd2O3 induced EMT in 16HBE cells and SD rat lung tissues. This was characterised by a decrease in epithelial markers and an increase in mesenchymal markers. Additionally, circ_009773 expression was found to decrease in 16HBE cells infected with NPs-Nd2O3 and also decreased in the lung tissues of SD rats. Relevant experiments showed that circ_009773 inhibited EMT in NPs-Nd2O3-treated 16HBE cells and SD rat lung tissues. The previous experiments revealed that circ_009773 was localised in the cytoplasm and functioned at the post-transcriptional level. With the EMT-related pathway used as the basis for circ_009773-related competing endogenous (ce)RNA mechanisms, our observations indicate that circ_009773 is capable of binding to and regulating the expression of miR-135b-5p. In summary, we found that circ_009773 inhibits the EMT induced by NPs-Nd2O3 in lung tissues, and this process likely occurs through competitive binding to miR-135b-5p.

Circ_009773在氧化钕纳米颗粒诱导肺上皮间质转化中的作用及潜在ceRNA鉴定。
氧化钕纳米颗粒(NPs-Nd2O3)通过呼吸道进入机体时可诱发呼吸系统相关疾病,包括肺组织损伤。然而,目前尚不清楚它们是否能诱导肺组织上皮-间质转化(EMT)及其相关机制。本研究通过体内和体外实验研究了circ_009773在NPs-Nd2O3诱导肺组织EMT过程中的作用。结果表明,NPs-Nd2O3可诱导16HBE细胞和SD大鼠肺组织发生EMT。其特征是上皮标记物减少,间充质标记物增加。此外,circ_009773在NPs-Nd2O3感染的16HBE细胞中表达降低,在SD大鼠肺组织中表达也降低。相关实验表明,circ_009773对nps - nd2o3处理的16HBE细胞和SD大鼠肺组织的EMT有抑制作用。先前的实验表明circ_009773定位于细胞质中,并在转录后水平起作用。emt相关通路被用作circ_009773相关竞争内源性(ce)RNA机制的基础,我们的观察表明circ_009773能够结合并调节miR-135b-5p的表达。综上所述,我们发现circ_009773抑制NPs-Nd2O3在肺组织中诱导的EMT,这一过程可能是通过与miR-135b-5p的竞争性结合发生的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Toxics
Toxics Chemical Engineering-Chemical Health and Safety
CiteScore
4.50
自引率
10.90%
发文量
681
审稿时长
6 weeks
期刊介绍: Toxics (ISSN 2305-6304) is an international, peer-reviewed, open access journal which provides an advanced forum for studies related to all aspects of toxic chemicals and materials. It publishes reviews, regular research papers, and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in detail. There is, therefore, no restriction on the maximum length of the papers, although authors should write their papers in a clear and concise way. The full experimental details must be provided so that the results can be reproduced. Electronic files or software regarding the full details of calculations and experimental procedure can be deposited as supplementary material, if it is not possible to publish them along with the text.
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