Zero-echo time imaging achieves whole brain activity mapping without ventral signal loss in mice

IF 4.7 2区医学 Q1 NEUROIMAGING

NeuroImage Pub Date : 2025-01-11 DOI:10.1016/j.neuroimage.2025.121024

Ayako Imamura , Rikita Araki , Yukari Takahashi , Koichi Miyatake , Fusao Kato , Sakiko Honjoh , Tomokazu Tsurugizawa

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引用次数: 0

Abstract

Functional MRI (fMRI) is an important tool for investigating functional networks. However, the widely used fMRI with T2*-weighted imaging in rodents has the problem of signal lack in the lateral ventral area of forebrain including the amygdala, which is essential for not only emotion but also noxious pain. Here, we scouted the zero-echo time (ZTE) sequence, which is robust to magnetic susceptibility and motion-derived artifacts, to image activation in the whole brain including the amygdala following the noxious stimulation to the hind paw. ZTE exhibited higher temporal signal-to-noise ratios than conventional fMRI sequences. Electrical sensory stimulation of the hind paw evoked ZTE signal increase in the primary somatosensory cortex. Formalin injection into the hind paw evoked early and latent change of ZTE signals throughout the whole brain including the subregions of amygdala. Furthermore, resting-state fMRI using ZTE demonstrated the functional connectivity, including that of the amygdala. These results indicate the feasibility of ZTE for whole brain fMRI including the amygdala and we first show acute and latent activity in different subnuclei of the amygdala complex after nociceptive stimulation.

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零回波时间成像在无腹侧信号丢失的情况下实现了小鼠全脑活动映射。

功能磁共振成像（fMRI）是研究功能网络的重要工具。然而，在啮齿类动物中广泛使用的fMRI T2*加权成像存在包括杏仁核在内的前脑外侧腹侧区信号缺乏的问题，而杏仁核不仅对情绪，而且对有害疼痛都是必不可少的。在这里，我们研究了零回波时间（ZTE）序列，它对磁化率和运动衍生伪影具有鲁棒性，对整个大脑（包括杏仁核）在后爪受到有害刺激后的图像激活具有鲁棒性。中兴通讯表现出比传统fMRI序列更高的空间和时间信噪比。后爪电刺激引起初级体感皮层中兴信号增加。后爪注射福尔马林可引起包括杏仁核亚区在内的全脑中兴信号的早期和潜伏性变化。此外，使用中兴通讯的静息状态fMRI显示了包括杏仁核在内的功能连接。这些结果表明中兴通讯在包括杏仁核在内的全脑fMRI上的可行性，我们首次在杏仁核复合体的不同亚核中显示了伤害性刺激后的急性和潜伏性活动。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

NeuroImage 医学-核医学

CiteScore

11.30

自引率

10.50%

发文量

809

审稿时长

63 days

期刊介绍： NeuroImage, a Journal of Brain Function provides a vehicle for communicating important advances in acquiring, analyzing, and modelling neuroimaging data and in applying these techniques to the study of structure-function and brain-behavior relationships. Though the emphasis is on the macroscopic level of human brain organization, meso-and microscopic neuroimaging across all species will be considered if informative for understanding the aforementioned relationships.