Transcriptome Analysis of the Harmful Dinoflagellate Heterocapsa bohaiensis Under Varied Nutrient Stress Conditions.

IF 4.1 2区 生物学 Q2 MICROBIOLOGY
Peng Peng, Fangxin Han, Xue Gong, Xiangyuan Guo, Ying Su, Yiwen Zhang, Jingjing Zhan
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Abstract

The increasing prevalence of harmful algal blooms (HABs) driven by eutrophication, particularly in China's nearshore waters, is a growing concern. Dinoflagellate Heterocapsa bohaiensis blooms have caused significant ecological and economic damage, as well as mass mortality, in cultivated species. Nutrients are one of the primary inducers of H. bohaiensis blooms. However, the transcriptomic studies of H. bohaiensis remain sparse, and its metabolic pathways are unknown. This study analyzed the transcriptome of H. bohaiensis under varying nutrient conditions (nitrogen at 128, 512, and 880 μM; phosphate at 8, 6, and 32 μM), focusing on differential gene expression. The results indicated that deviations in nutrient conditions (higher or lower N:P ratios) led to a higher number of differentially expressed genes compared to the control (N:P ratios = 27.5), thereby underscoring their pivotal role in growth. Gene Ontology (GO) enrichment analyses showed that nutrient limitation upregulated the biosynthesis and catabolism processes while downregulating the cell cycle and division functions. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that, under nitrogen limitation, the proteasome pathways were upregulated, while photosynthesis and carbon fixation were downregulated; under phosphorus limitation, the proteasome pathways were upregulated and nitrogen metabolism was downregulated. These findings suggest that H. bohaiensis adapts to nutrient stress by adjusting its metabolic processes.

不同营养胁迫条件下渤海异藻有害鞭毛藻转录组分析。
富营养化导致的有害藻华(HABs)日益普遍,特别是在中国近岸水域,这是一个越来越令人担忧的问题。渤海异鞭藻(Heterocapsa bohaiensis)在养殖物种中造成了严重的生态和经济破坏和大量死亡。营养物是海南赤潮的主要诱导因子之一。然而,对海南人的转录组学研究仍然很少,其代谢途径尚不清楚。本研究分析了不同营养条件(氮浓度为128、512和880 μM;磷酸盐在8、6和32 μM),重点关注差异基因表达。结果表明,与对照(N:P比率= 27.5)相比,营养条件的偏差(较高或较低的N:P比率)导致差异表达基因的数量增加,从而强调了它们在生长中的关键作用。基因本体(Gene Ontology, GO)富集分析表明,营养限制上调了生物合成和分解代谢过程,同时下调了细胞周期和分裂功能。京都基因与基因组百科(KEGG)分析显示,氮素限制下,蛋白酶体途径上调,光合作用和固碳途径下调;在限磷条件下,蛋白酶体途径上调,氮代谢下调。这些结果表明,海南猿猴通过调节其代谢过程来适应营养胁迫。
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来源期刊
Microorganisms
Microorganisms Medicine-Microbiology (medical)
CiteScore
7.40
自引率
6.70%
发文量
2168
审稿时长
20.03 days
期刊介绍: Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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