Maria Guadalupe Castillo-Texta, José Augusto Ramírez-Trujillo, Edgar Dantán-González, Mario Ramírez-Yáñez, Ramón Suárez-Rodríguez
{"title":"Endophytic Bacteria from the Desiccation-Tolerant Plant <i>Selaginella lepidophylla</i> and Their Potential as Plant Growth-Promoting Microorganisms.","authors":"Maria Guadalupe Castillo-Texta, José Augusto Ramírez-Trujillo, Edgar Dantán-González, Mario Ramírez-Yáñez, Ramón Suárez-Rodríguez","doi":"10.3390/microorganisms12122654","DOIUrl":null,"url":null,"abstract":"<p><p>Bacteria associated with plants, whether rhizospheric, epiphytic, or endophytic, play a crucial role in plant productivity and health by promoting growth through complex mechanisms known as plant growth promoters. This study aimed to isolate, characterize, identify, and evaluate the potential of endophytic bacteria from the resurrection plant <i>Selaginella lepidophylla</i> in enhancing plant growth, using <i>Arabidopsis thaliana</i> ecotype Col. 0 as a model system. Plant growth-promotion parameters were assessed on the bacterial isolates; this assessment included the quantification of indole-3-acetic acid, phosphate solubilization, and biological nitrogen fixation, a trehalose quantification, and the siderophore production from 163 endophytic bacteria isolated from <i>S. lepidophylla</i>. The bacterial genera identified included <i>Agrobacterium</i>, <i>Burkholderia</i>, <i>Curtobacterium</i>, <i>Enterobacter</i>, <i>Erwinia</i>, <i>Pantoea</i>, <i>Pseudomonas</i>, and <i>Rhizobium</i>. The plant growth promotion in <i>A. thaliana</i> was evaluated both in Murashige Skoog medium, agar-water, and direct seed inoculation. The results showed that the bacterial isolates enhanced primary root elongation and lateral root and root hair development, and increased the fresh and dry biomass. Notably, three isolates promoted early flowering in <i>A. thaliana</i>. Based on these findings, we propose the <i>S. lepidophylla</i> bacterial isolates as ideal candidates for promoting growth in other agriculturally important plants.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1000,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microorganisms","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/microorganisms12122654","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Bacteria associated with plants, whether rhizospheric, epiphytic, or endophytic, play a crucial role in plant productivity and health by promoting growth through complex mechanisms known as plant growth promoters. This study aimed to isolate, characterize, identify, and evaluate the potential of endophytic bacteria from the resurrection plant Selaginella lepidophylla in enhancing plant growth, using Arabidopsis thaliana ecotype Col. 0 as a model system. Plant growth-promotion parameters were assessed on the bacterial isolates; this assessment included the quantification of indole-3-acetic acid, phosphate solubilization, and biological nitrogen fixation, a trehalose quantification, and the siderophore production from 163 endophytic bacteria isolated from S. lepidophylla. The bacterial genera identified included Agrobacterium, Burkholderia, Curtobacterium, Enterobacter, Erwinia, Pantoea, Pseudomonas, and Rhizobium. The plant growth promotion in A. thaliana was evaluated both in Murashige Skoog medium, agar-water, and direct seed inoculation. The results showed that the bacterial isolates enhanced primary root elongation and lateral root and root hair development, and increased the fresh and dry biomass. Notably, three isolates promoted early flowering in A. thaliana. Based on these findings, we propose the S. lepidophylla bacterial isolates as ideal candidates for promoting growth in other agriculturally important plants.
期刊介绍:
Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.