Differences in the Microbial Composition and Function of the Arundo donax Rhizosphere Under Different Cultivation Conditions.

IF 4.1 2区 生物学 Q2 MICROBIOLOGY
Fan Yang, Miaomiao Liu, Xin Wang, Yuting Hong, Qiuju Yao, Xiaoke Chang, Gongyao Shi, Weiwei Chen, Baoming Tian, Abeer Hegazy
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Abstract

Rhizosphere microorganisms play an important role in the health and development of root systems. Investigating the microbial composition of the rhizosphere is central to understanding the inter-root microbial function of Arundo donax under various cultivation conditions. To complement the metagenomic study of the Arundo donax rhizosphere, here, an amplicon-based metagenomic survey of bacteria and fungi was selected as a practical approach to analyzing the abundance, diversity index, and community structure of rhizosphere bacteria and fungi, as well as to study the effects of different cultivation methods on rhizosphere microbial diversity. Next-generation sequencing and QIIME2 analysis were used. The results indicated that microbial community richness, diversity, and evenness of the hydroponic samples were lower than those of soil samples when examining the α diversity indices of bacteria and fungi using Chao1, ACE, and Shannon metrics. In particular, the relative abundances of Proteobacteria, Rhizobiales, and Incertae sedis in hydroponic materials were higher, while Basidiomycota, Ascomycota, and Actinobacteriota dominated the flora in soil materials when comparing the numbers of OTUs and the ACE community richness estimator. Furthermore, the rhizosphere of hydroponic A. donax contained a higher abundance of nitrogen-fixing bacteria and photosynthetic bacteria, which contribute to root formation. Additionally, there was a significant presence of Basidiomycota, Ascomycota, and Actinobacteriota in soil A. donax, which can form hyphae. This reveals that the microbial community composition of the A. donax rhizosphere is significantly different under various cultivation conditions, suggesting that employing two distinct culturing techniques for Arundo donax may alter the microbiome. Furthermore, it provides technical support for the synergistic interaction between Arundo donax and rhizosphere microorganisms so as to better use the relationship between Arundo donax and basic microorganisms to solve the problems of Arundo donax growth and ecological restoration.

不同栽培条件下芦竹根际微生物组成及功能的差异
根际微生物对根系的健康和发育起着重要的作用。研究根际微生物组成是了解不同栽培条件下芦笋根际微生物功能的关键。为了补充对芦竹根际微生物的宏基因组研究,本文采用基于扩增子的细菌和真菌宏基因组调查方法,分析了芦竹根际细菌和真菌的丰度、多样性指数和群落结构,并研究了不同培养方法对芦竹根际微生物多样性的影响。采用新一代测序和QIIME2分析。结果表明,采用Chao1、ACE和Shannon指标测定细菌和真菌的α多样性指数时,水培样品的微生物群落丰富度、多样性和均匀性均低于土壤样品。其中,Proteobacteria、Rhizobiales和Incertae setis在水培材料中的相对丰度较高,而担子菌门(Basidiomycota)、子囊菌门(Ascomycota)和放线菌门(Actinobacteriota)在土壤材料中占主导地位。此外,水培唐草根际中含有丰富的固氮细菌和光合细菌,这些细菌有助于根的形成。此外,土壤中有大量担子菌、子囊菌和放线菌,它们可以形成菌丝。这表明,不同栽培条件下黄杨根际微生物群落组成存在显著差异,表明采用两种不同的栽培技术可能会改变黄杨根际微生物组。进一步为芦笋与根际微生物的协同互作提供技术支持,从而更好地利用芦笋与基础微生物的关系,解决芦笋生长和生态恢复问题。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microorganisms
Microorganisms Medicine-Microbiology (medical)
CiteScore
7.40
自引率
6.70%
发文量
2168
审稿时长
20.03 days
期刊介绍: Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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