Molecular Testing of Zoonotic Bacteria in Cattle, Sheep, and Goat Abortion Cases in Botswana.

IF 4.1 2区 生物学 Q2 MICROBIOLOGY
Boitumelo M Modise-Tlotleng, Sununguko W Mpoloka, Tirumala B K Settypalli, Joseph Hyera, Tebogo Kgotlele, Kago Kumile, Mosarwa E Sechele, Obuile O Raboloko, Chandapiwa Marobela-Raborokgwe, Gerrit J Viljoen, Giovanni Cattoli, Charles E Lamien
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Abstract

Abortion is one of the major causes of economic losses in livestock production worldwide. Because several factors can lead to abortion in cattle, sheep and goats, laboratory diagnosis, including the molecular detection of pathogens causing abortion, is often necessary. Bacterial zoonotic diseases such as brucellosis, coxiellosis, leptospirosis, and listeriosis have been implicated in livestock abortion, but they are under diagnosed and under-reported in most developing countries, including Botswana. This study applied a recently developed multiplex high-resolution melting analysis technique, coupled with singleplex qPCR assays, to investigate abortions in livestock in Botswana, using 152 samples from cattle, sheep, and goat abortion cases. Brucella spp. were the most frequent pathogen detected, with an overall frequency of 21.1%, followed by Coxiella burnetii with 19.1%. Listeria monocytogenes and Leptospira spp. were not detected in any of specimens samples investigated. Mixed infections with Brucella spp. and C. burnetii were observed in 35% specimes examined. There was a good agreement between the multiplex qPCR-HRM and singleplex qPCR for detecting Brucella spp. and C. burnetii. This study is the first report on the syndromic testing of abortion-causing pathogens in Botswana. It shows the importance of molecular methods in the differential diagnosis of abortion-causing diseases in domestic ruminants.

博茨瓦纳牛、绵羊和山羊流产病例中人畜共患病细菌的分子检测。
流产是全世界畜牧生产经济损失的主要原因之一。由于多种因素可导致牛、绵羊和山羊流产,因此经常需要进行实验室诊断,包括对导致流产的病原体进行分子检测。细菌性人畜共患疾病,如布鲁氏菌病、克氏菌病、钩端螺旋体病和李斯特菌病与牲畜流产有关,但在包括博茨瓦纳在内的大多数发展中国家,这些疾病的诊断和报告都不足。本研究应用了最近开发的多重高分辨率熔融分析技术,结合单重qPCR分析,对博茨瓦纳牲畜流产进行了调查,使用了152个牛、绵羊和山羊流产案例样本。布鲁氏菌是最常见的病原菌,总检出率为21.1%,其次是伯纳氏杆菌,总检出率为19.1%。在调查的所有标本中均未检出单核增生李斯特菌和钩端螺旋体。在35%的标本中观察到布鲁氏菌和伯氏菌的混合感染。多重qPCR- hrm与单一qPCR检测布鲁氏菌和伯氏菌的结果吻合较好。本研究是博茨瓦纳流产致病菌综合征检测的第一份报告。说明分子方法在家畜反刍动物流产疾病鉴别诊断中的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microorganisms
Microorganisms Medicine-Microbiology (medical)
CiteScore
7.40
自引率
6.70%
发文量
2168
审稿时长
20.03 days
期刊介绍: Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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