Genome Analysis of Coxsackievirus A4 Identified from Herpangina Children in Northern China.

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2025-01-13 DOI:10.1007/s00284-025-04075-7

Tiegang Lv, Yingming Li, Pengfei You, Xiaoyan Chen, Huafeng Li, Na Zhang, Chunling Ma, Yanli Zhang, Jie Chen

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Abstract

Sporadic epidemics of coxsackievirus A4 (CVA4) have been reported worldwide. However, the lack of the whole genome sequence has restricted the study of the gene characterization and evolution of CVA4. In this study, four whole genome sequences and 17 VP1 sequences of CVA4 identified from Linyi, northern China, in summer 2024 were used for genetic characterization and phylogenetic analysis. Four genotypes (A, B, C, and D) and five subgenotypes (C1-C5) were identified based on VP1 sequences. The Linyi CVA4 strains belong to subgenotype C2, which has also been the main prevalent subgenotype in China in recent years. The Linyi CVA4 strains exhibited high homology with the CVA4 prototype strain in the P1 region while exhibited higher homology with some non-CVA4 EV-A strains identified in China, including five CVA2 strains, three CVA5 strains, three CVA6 strains, one CVA8 strain, one CVA12 strain, and one CVA14 strain in the P2 and P3 regions. Recombination analysis of the whole genome sequences of the Linyi CV4 strains revealed that two Linyi CVA4 strains might be recombinants of one Shanghai CVA4 strain (KJ541163) and one Jiangsu CVA2 strain (OL519580). One Linyi CVA4 strain might be a recombinant of one Shandong CVA2 strain (MK967660) and one Shanghai CVA4 strain (KJ541163).

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中国北方赫氏咽峡病患儿柯萨奇病毒A4的基因组分析

柯萨奇病毒A4 （CVA4）的散发流行已在世界范围内报道。然而，由于CVA4全基因组序列的缺乏，限制了CVA4基因表征和进化的研究。本研究利用2024年夏季在中国北方临沂鉴定的4个CVA4全基因组序列和17个VP1序列进行了遗传鉴定和系统发育分析。基于VP1序列鉴定出4个基因型（A、B、C、D）和5个亚基因型（c1 ~ c5）。临沂CVA4菌株属C2亚基因型，也是近年来中国流行的主要亚基因型。临沂CVA4株在P1区与CVA4原型株具有较高的同源性，与国内已发现的非CVA4 EV-A株具有较高的同源性，包括P2和P3区5株CVA2、3株CVA5、3株CVA6、1株CVA8、1株CVA12和1株CVA14。对临沂CVA4株全基因组序列的重组分析表明，2株临沂CVA4可能是1株上海CVA4 （KJ541163）和1株江苏CVA2 （OL519580）的重组体。1株临沂CVA4可能是由1株山东CVA2 （MK967660）和1株上海CVA4 （KJ541163）重组而成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.