Plasmon-Driven Gold Nanopillar Multiarrayed Gene Amplification Methodology for the High-Throughput Discrimination of Pathogens

IF 14.3 1区 材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Sung Eun Seo, Kyung Ho Kim, Seo Jin Kim, Kyong-Cheol Ko, Woo-Keun Kim, Kyoung G. Lee, Oh Seok Kwon
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Abstract

Molecular diagnosis limitations, including complex treatment processes, low cost-effectiveness, and operator-dependent low reproducibility, interrupt the timely prevention of disease spread and the development of medical devices for home and outdoor uses. A newly fabricated gold nanopillar array-based film is presented for superior photothermal energy conversion. Magnifying the metal film surface-to-volume ratio increases the photothermal energy conversion efficiency, resulting in a swift reduction in the gene amplification reaction time. Plasmonic energy-based ultrafast gene amplification and facile confirmation methodology offer a rapid disease discrimination platform for high-throughput multiplexed diagnosis. The superior performance of the gold nanopillar arrayed film is demonstrated by measuring the amount of pathogen (Vibrio cholerae) with a sensitivity of 101 cfu mL−1 in 5.5 min. The newly engineered gold nanopillar arrayed film can be utilized to diagnose universal pathogens to achieve an increasingly successful complete cure.

Abstract Image

等离子体驱动的金纳米柱多阵列基因扩增方法用于病原体的高通量鉴别。
分子诊断的局限性,包括复杂的治疗过程、低成本效益和依赖操作者的低重复性,妨碍了疾病传播的及时预防和家用和户外医疗设备的开发。提出了一种新型的基于金纳米柱阵列的光热转换薄膜。放大金属膜的表面体积比,提高光热能量转换效率,使基因扩增反应时间迅速缩短。基于等离子体能量的超快速基因扩增和简易确认方法为高通量多路诊断提供了快速的疾病鉴别平台。通过在5.5 min内检测病原菌(霍乱弧菌)的数量,证明了金纳米柱排列膜的优越性能,其灵敏度为101 cfu mL-1。新设计的金纳米柱排列膜可用于诊断通用病原体,从而越来越成功地实现完全治愈。
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来源期刊
Advanced Science
Advanced Science CHEMISTRY, MULTIDISCIPLINARYNANOSCIENCE &-NANOSCIENCE & NANOTECHNOLOGY
CiteScore
18.90
自引率
2.60%
发文量
1602
审稿时长
1.9 months
期刊介绍: Advanced Science is a prestigious open access journal that focuses on interdisciplinary research in materials science, physics, chemistry, medical and life sciences, and engineering. The journal aims to promote cutting-edge research by employing a rigorous and impartial review process. It is committed to presenting research articles with the highest quality production standards, ensuring maximum accessibility of top scientific findings. With its vibrant and innovative publication platform, Advanced Science seeks to revolutionize the dissemination and organization of scientific knowledge.
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