Plasmon-Driven Gold Nanopillar Multiarrayed Gene Amplification Methodology for the High-Throughput Discrimination of Pathogens.

Abstract

Molecular diagnosis limitations, including complex treatment processes, low cost-effectiveness, and operator-dependent low reproducibility, interrupt the timely prevention of disease spread and the development of medical devices for home and outdoor uses. A newly fabricated gold nanopillar array-based film is presented for superior photothermal energy conversion. Magnifying the metal film surface-to-volume ratio increases the photothermal energy conversion efficiency, resulting in a swift reduction in the gene amplification reaction time. Plasmonic energy-based ultrafast gene amplification and facile confirmation methodology offer a rapid disease discrimination platform for high-throughput multiplexed diagnosis. The superior performance of the gold nanopillar arrayed film is demonstrated by measuring the amount of pathogen (Vibrio cholerae) with a sensitivity of 10¹ cfu mL^-1 in 5.5 min. The newly engineered gold nanopillar arrayed film can be utilized to diagnose universal pathogens to achieve an increasingly successful complete cure.