Tomato Defenses Under Stress: The Impact of Salinity on Direct Defenses Against Insect Herbivores.

Abstract

Abiotic stressors, such as salt stress, can reduce crop productivity, and when combined with biotic pressures, such as insect herbivory, can exacerbate yield losses. However, salinity-induced changes to plant quality and defenses can in turn affect insect herbivores feeding on plants. This study investigates how salinity stress in tomato plants (Solanum Lycopersicum cv. Better Boy) impacts the behavior and performance of a devastating insect pest, the tomato fruitworm caterpillar (Helicoverpa zea). Through choice assays and performance experiments, we demonstrate that salt-stressed tomato plants are poor hosts for H. zea, negatively affecting caterpillar feeding preferences and growth rates. While changes in plant nutritional quality were observed, the primary factor influencing insect performance appears to be direct ionic toxicity, which significantly impairs multiple life history parameters of H. zea including survival, pupation, adult emergence, and fecundity. Plant defense responses show complex interactions between salt stress and herbivory, with two proteinase inhibitor genes - PIN2 and AspPI, showing a higher induced response to insect herbivory under salt conditions. However, plant defenses do not seem to be the main driver of reduced caterpillar performance on salt-treated plants. Furthermore, we report reduced oviposition by H. zea moths on salt-treated plants, which was correlated with altered volatile emissions. Our findings reveal that H. zea exhibits optimal host selection behaviours for both larval feeding and adult oviposition decisions, which likely contribute to its success as an agricultural pest. This research provides insights into the complex interactions between abiotic stress, plant physiology, and insect behaviour, with potential implications for pest management strategies in saline agricultural environments.