A SERS/colorimetric biosensor based on AuNSs@Ag core–shell Prussian blue nanozyme for non-interference and rapid detection of Staphylococcus aureus in milk

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-01-15 DOI:10.1007/s00604-024-06921-0

Yajun Zhao, Ming Guan, Fang Mi, Yiyao Zhang, Pengfei Geng, Shan Zhang, Han Song, Guotong Chen

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Abstract

A AuNSs@PB@Ag-Apt surface-enhanced Raman scattering (SERS) probe has been developed by embedding Prussian blue (PB) between Au core and Ag shell. The PB SERS probe illustrates strong SERS activity in the Raman silent region of 2070 cm⁻¹, and has a zero background signal, ensuring high sensitivity for the detection of Staphylococcus aureus (S. aureus). Moreover, the assemble SERS probe exhibits the catalytic function of peroxidase (POD), enabling the catalysis of 3,3′,5,5′-tetramethylbenzidine (TMB) for colorimetric detection. Additionally, we prepared lectin-functionalized magnetic nanoparticles (F-MNPs-ConA) that demonstrate superior adsorption capabilities towards S. aureus, achieving an impressive adsorption value of up to 98.62%. The F-MNPs-ConA/S. aureus/AuNSs@PB@Ag-Apt sandwich structure constructed by the above SERS probe and magnetic nanoparticles achieves highly sensitive detection of S. aureus. The detection range is 1 × 10⁰ ~ 1 × 10⁸ CFU·mL⁻¹, and the detection limit (LOD) is 1 CFU·mL⁻¹. Notably, this method can be integrated into smartphones, facilitating rapid and on-site detection of S. aureus. Compared with similar biosensors, this biosensor offers distinct advantages, including a wide detection range and a low detection limit. The reliability of the biosensor was confirmed by its application in milk, where the recovery ranged from 93.66 to 120.8%. The dual-mode SERS/colorimetric detection, non-interfering nature, magnetic enrichment, and reusability of the biosensor make it a promising tool for point-of-care testing (POCT).

Graphical Abstract

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基于AuNSs@Ag核壳普鲁士蓝纳米酶的SERS/比色生物传感器用于牛奶中金黄色葡萄球菌的无干扰快速检测

利用普鲁士蓝（PB）包埋在Au核和Ag壳层之间，研制了AuNSs@PB@Ag-Apt表面增强拉曼散射（SERS）探针。PB SERS探针在2070 cm−1的拉曼沉默区具有较强的SERS活性，并且具有零背景信号，确保了对金黄色葡萄球菌（S. aureus）检测的高灵敏度。此外，组装SERS探针具有过氧化物酶（POD）的催化功能，可以催化3,3 ',5,5 ' -四甲基联苯胺（TMB）进行比色检测。此外，我们制备的凝集素功能化磁性纳米颗粒（F-MNPs-ConA）对金黄色葡萄球菌表现出优异的吸附能力，吸附值高达98.62%。F-MNPs-ConA / S。上述SERS探针与磁性纳米颗粒构建的ag - apt三明治结构实现了对金黄色葡萄球菌的高灵敏度检测。检测范围为1 × 100 ~ 1 × 108 CFU·mL−1，检出限（LOD）为1 CFU·mL−1。值得注意的是，该方法可以集成到智能手机中，方便快速和现场检测金黄色葡萄球菌。与同类生物传感器相比，该生物传感器具有明显的优势，包括检测范围宽，检测极限低。该传感器在牛奶中的回收率为93.66 ~ 120.8%，验证了该传感器的可靠性。双模SERS/比色检测、无干扰性、磁富集和可重复使用的生物传感器使其成为一种有前途的点护理检测（POCT）工具。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.