Visual detection of kanamycin with functionalized Au nanoparticles

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-01-14 DOI:10.1007/s00604-025-06954-z

Xigui Liu, Danlian Huang, Cui Lai, Fei Wang

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Abstract

A simple and rapid colorimetric detection strategy, based on hydrogen bond identification of 6-thioguanine (6-TG) functionalized Au nanoparticles (AuNPs), is proposed for highly selective and sensitive determination of kanamycin (KA). In this strategy, the hydrogen bond interaction between 6-TG and kanamycin induces AuNPs to agglomerate, with a consequent color change of AuNPs from wine red to purple or even blue. The kanamycin concentrations can be quantified by employing UV–vis spectrophotometer. The results display that kanamycin concentrations (0.005 to 18 µM) are linearly related to A₆₂₀/A₅₂₀ (the absorbance ratio of 620 nm and 520 nm) with a LOD of 1.8 nM and a LOQ of 5.9 nM (S/N = 3). This strategy also reveals a high degree of selectivity among a series of common interfering species. Moreover, the strategy can be employed to detect trace amounts of kanamycin in real-life samples, and it shows satisfying results compared with high performance liquid chromatography. In general, this developed strategy is facile and inexpensive without the need for complex processing procedures and expensive instruments. In addition, this work may further exploit detection strategies for other organic contaminants, as well as make a strong contribution to the development of the colorimetric method.

Graphical Abstract

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功能化金纳米颗粒对卡那霉素的目视检测

基于氢键鉴定的6-硫鸟嘌呤（6-TG）功能化金纳米粒子（AuNPs），提出了一种高选择性、高灵敏度的卡那霉素（KA）的快速比色检测策略。在这种策略中，6-TG和卡那霉素之间的氢键相互作用诱导AuNPs聚集，从而使AuNPs的颜色从酒红色变为紫色甚至蓝色。用紫外-可见分光光度计测定卡那霉素的浓度。结果表明，卡那霉素浓度（0.005 ~ 18µM）与A620/A520（吸光度分别为620 nm和520 nm）呈线性相关，LOD为1.8 nm， LOQ为5.9 nm （S/N = 3）。该策略还揭示了对一系列常见干扰物种的高度选择性。此外，该策略可用于实际样品中痕量卡那霉素的检测，并与高效液相色谱法相比显示出令人满意的结果。总的来说，这种已开发的策略既方便又便宜，不需要复杂的处理程序和昂贵的仪器。此外，该工作还可以进一步开发其他有机污染物的检测策略，并为比色法的发展做出重大贡献。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.