The aerial epidermis is a major site of quinolizidine alkaloid biosynthesis in narrow-leafed lupin

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2025-01-14 DOI:10.1111/nph.20384

Karen Michiko Frick, Marcus Daniel Brandbjerg Bohn Lorensen, Nikola Micic, Eddi Esteban, Asher Pasha, Alexander Schulz, Nicholas James Provart, Hussam Hassan Nour-Eldin, Nanna Bjarnholt, Christian Janfelt, Fernando Geu-Flores

引用次数: 0

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气生表皮是狭叶羽扇豆喹嗪类生物碱生物合成的主要场所

羽扇豆是一种很有前途的蛋白质作物，其种子中积累有毒的喹诺齐啶生物碱（QAs），使其最终用途复杂化。QAs在绿色器官（叶、茎和豆荚）中合成，其中一部分在果实发育过程中被运输到种子中。生物合成和积累的确切位置尚不清楚；然而，叶肉细胞被认为是来源，表皮细胞被认为是汇。我们利用基于质谱成像（MSI）、激光捕获显微解剖结合RNA - Seq、前体饲养结合LC - MS和MSI的研究方法，研究了QA在窄叶lupin （Lupinus angustifolius）生物合成器官中合成和积累的确切位点。我们发现积累在种子中的QAs（“核心”QAs）在组织中均匀分布；然而，它们的酯化版本主要积聚在表皮。令人惊讶的是，RNA‐Seq揭示了表皮中强烈的生物合成基因表达，并通过定量实时聚合酶链反应在叶片中证实了这一点。最后，使用稳定标记的前体进行饲养研究表明，下叶表皮是高度生物合成的。我们的研究结果表明，表皮是窄叶扁豆QA生物合成的主要部位，挑战了目前的假设。我们的工作对阐明QA生物合成途径和从来源到种子的长距离运输网络具有直接意义。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.