Oreochromicin-2 shows antimicrobial and immunostimulant effect against respiratory pathogens in pigs

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2024-12-26 DOI:10.1016/j.rvsc.2024.105523

Liliana Basabe-Tuero , Lázara Ayala , Ivette Espinosa , Yeleiny Machín , Liany Coto , Carlos Duarte , Soraya Piloto , Antonio Morales , Osmany Rodrigo , David Diago , Iliana Sosa , Mario Pablo Estrada , Rebeca Martínez

{"title":"Oreochromicin-2 shows antimicrobial and immunostimulant effect against respiratory pathogens in pigs","authors":"Liliana Basabe-Tuero , Lázara Ayala , Ivette Espinosa , Yeleiny Machín , Liany Coto , Carlos Duarte , Soraya Piloto , Antonio Morales , Osmany Rodrigo , David Diago , Iliana Sosa , Mario Pablo Estrada , Rebeca Martínez","doi":"10.1016/j.rvsc.2024.105523","DOIUrl":null,"url":null,"abstract":"<div><div>Porcine respiratory diseases have a huge economic impact on pig production. The highest incidence of these diseases is commonly linked to <em>Streptococcus suis</em>, <em>Pasteurella multocida</em> and <em>Bordetella bronchiseptica</em>, some of which are zoonotic posing a risk to human health. The inappropriate and excessive use of conventional antibiotics, as usual procedure for treating respiratory diseases in pigs, has generated the emergence of antimicrobial resistance (AMR), which urgently requires the development of alternative approaches to current antimicrobials. Antimicrobial peptides (AMPs) have rapidly garnered interest as novel therapeutic candidates. Oreochromicin-2 (Oreoch-2), an AMP previously isolated from <em>Oreochromis niloticus</em> gills, has shown broad antibacterial properties against several species. However, studies about its effect on porcine respiratory pathogens and its potential use for the treatment of swine respiratory diseases are not available. In this work we determined the <em>in vitro</em> antimicrobial activity of the peptide against <em>S. suis</em> by a broth microdilution method. Oreoch-2 showed a MIC of 3.13 μM against this pathogen. For <em>in vivo</em> experiments, Yorkshire x Landrace crossbred (LYxL35) weaning pigs aged 30–33 days were allocated in pens containing ten pigs each. To study the influence of the peptide on health status, a total clinical score was determined. The administration of Oreoch-2 improved the clinical behavior of the animals, similar to the conventional antibiotic shotapen, respect to the placebo group. A reduction of bacterial loads in the respiratory tract and lungs was observed in Oreoch-2-treated animals as compared to the placebo group. It was shown that peptide treated-piglets displayed significantly higher serum IgG concentration compared to the control group. These results demonstrated Oreoch-2 potential as an antimicrobial and immunostimulant drug candidate against respiratory diseases in pigs.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"184 ","pages":"Article 105523"},"PeriodicalIF":2.2000,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Research in veterinary science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0034528824003904","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Porcine respiratory diseases have a huge economic impact on pig production. The highest incidence of these diseases is commonly linked to Streptococcus suis, Pasteurella multocida and Bordetella bronchiseptica, some of which are zoonotic posing a risk to human health. The inappropriate and excessive use of conventional antibiotics, as usual procedure for treating respiratory diseases in pigs, has generated the emergence of antimicrobial resistance (AMR), which urgently requires the development of alternative approaches to current antimicrobials. Antimicrobial peptides (AMPs) have rapidly garnered interest as novel therapeutic candidates. Oreochromicin-2 (Oreoch-2), an AMP previously isolated from Oreochromis niloticus gills, has shown broad antibacterial properties against several species. However, studies about its effect on porcine respiratory pathogens and its potential use for the treatment of swine respiratory diseases are not available. In this work we determined the in vitro antimicrobial activity of the peptide against S. suis by a broth microdilution method. Oreoch-2 showed a MIC of 3.13 μM against this pathogen. For in vivo experiments, Yorkshire x Landrace crossbred (LYxL35) weaning pigs aged 30–33 days were allocated in pens containing ten pigs each. To study the influence of the peptide on health status, a total clinical score was determined. The administration of Oreoch-2 improved the clinical behavior of the animals, similar to the conventional antibiotic shotapen, respect to the placebo group. A reduction of bacterial loads in the respiratory tract and lungs was observed in Oreoch-2-treated animals as compared to the placebo group. It was shown that peptide treated-piglets displayed significantly higher serum IgG concentration compared to the control group. These results demonstrated Oreoch-2 potential as an antimicrobial and immunostimulant drug candidate against respiratory diseases in pigs.

查看原文本刊更多论文

Oreochromicin-2对猪呼吸道病原体具有抗菌和免疫刺激作用。

猪呼吸道疾病对养猪生产造成巨大的经济影响。这些疾病的最高发病率通常与猪链球菌、多杀性巴氏杆菌和支气管脓毒杆菌有关，其中一些是人畜共患疾病，对人类健康构成威胁。作为治疗猪呼吸道疾病的常规程序，传统抗生素的不适当和过度使用已经产生了抗菌素耐药性（AMR），这迫切需要开发替代现有抗菌素的方法。抗菌肽（AMPs）已迅速引起人们的兴趣，作为新的治疗候选者。Oreochromicin-2 （Oreochromicin-2）是一种先前从nilochromis鳃中分离到的AMP，已显示出对多种物种的广泛抗菌性能。然而，关于其对猪呼吸道病原体的作用及其在猪呼吸道疾病治疗中的潜在应用的研究尚未得到证实。本研究采用肉汤微量稀释法测定了该肽对猪链球菌的体外抗菌活性。Oreoch-2对该病原菌的MIC为3.13 μM。在体内试验中，选用30-33日龄的约克郡与长白杂交断奶仔猪（LYxL35），每栏10头。为了研究多肽对健康状况的影响，测定了临床总评分。与安慰剂组相比，Oreoch-2改善了动物的临床行为，与常规抗生素shotapen类似。与安慰剂组相比，oreoch -2治疗的动物呼吸道和肺部细菌负荷减少。结果表明，肽处理仔猪血清IgG浓度显著高于对照组。这些结果表明Oreoch-2有潜力作为抗猪呼吸道疾病的抗微生物和免疫刺激性候选药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.