Genotoxicity evaluation of fucoidan-rich Undaria pinnatifida sporophyll.

IF 1.6 4区医学 Q4 TOXICOLOGY

Toxicological Research Pub Date : 2024-08-23 eCollection Date: 2025-01-01 DOI:10.1007/s43188-024-00258-9

Miran Jang, Soo-Im Choi, Gun-Hee Kim

{"title":"Genotoxicity evaluation of fucoidan-rich Undaria pinnatifida sporophyll.","authors":"Miran Jang, Soo-Im Choi, Gun-Hee Kim","doi":"10.1007/s43188-024-00258-9","DOIUrl":null,"url":null,"abstract":"The aim of this study was to investigate genotoxicity of fucoidan-rich Undaria pinnatifida sporophyll (FUPS) using a three-component test battery. Our sulfate analysis method showed that FUPS extract contained 14% fucoidan sulfate. The reverse mutation assay showed that the FUPS extract did not increase the number of revertant colonies in any of the five bacterial strains tested, regardless of metabolic activation by S9 mix. Furthermore, FUPS did not induce chromosomal aberrations in the 6-h short-term test with or without S9, as well as in the 24-h continuous test without S9. Finally, bone marrow micronucleus examination of ICR mice at oral doses up to 5000 mg/kg/day did not show a significantly dose-dependent increase in the frequency of micronucleated polychromatic erythrocytes (MNPCEs) or the ratio of polychromatic erythrocytes (PCEs) to total erythrocytes. In conclusion, it was determined that the FUPS extract does not have a significant genotoxic potential under the expected conditions of use.Supplementary information: The online version contains supplementary material available at 10.1007/s43188-024-00258-9.","PeriodicalId":23181,"journal":{"name":"Toxicological Research","volume":"41 1","pages":"39-46"},"PeriodicalIF":1.6000,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11717731/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Toxicological Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s43188-024-00258-9","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"TOXICOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The aim of this study was to investigate genotoxicity of fucoidan-rich Undaria pinnatifida sporophyll (FUPS) using a three-component test battery. Our sulfate analysis method showed that FUPS extract contained 14% fucoidan sulfate. The reverse mutation assay showed that the FUPS extract did not increase the number of revertant colonies in any of the five bacterial strains tested, regardless of metabolic activation by S9 mix. Furthermore, FUPS did not induce chromosomal aberrations in the 6-h short-term test with or without S9, as well as in the 24-h continuous test without S9. Finally, bone marrow micronucleus examination of ICR mice at oral doses up to 5000 mg/kg/day did not show a significantly dose-dependent increase in the frequency of micronucleated polychromatic erythrocytes (MNPCEs) or the ratio of polychromatic erythrocytes (PCEs) to total erythrocytes. In conclusion, it was determined that the FUPS extract does not have a significant genotoxic potential under the expected conditions of use.

Supplementary information: The online version contains supplementary material available at 10.1007/s43188-024-00258-9.

查看原文本刊更多论文

富岩藻胶酸的裙带菜孢子叶遗传毒性评价。

本研究采用三组份试验电池研究富岩藻胶酸的裙带菜孢子叶（FUPS）的遗传毒性。我们的硫酸盐分析方法表明，FUPS提取物中含有14%的岩藻糖聚糖硫酸盐。反向突变试验表明，无论S9混合物是否激活代谢，FUPS提取物都不会增加五种细菌菌株的逆转菌落数量。此外，在有或没有S9的6小时短期试验中，以及在没有S9的24小时连续试验中，FUPS均未引起染色体畸变。最后，口服剂量高达5000mg /kg/天的ICR小鼠骨髓微核检查未显示微核多染红细胞（mnpce）频率或多染红细胞（pce）与总红细胞比率的显着剂量依赖性增加。综上所述，在预期的使用条件下，确定了FUPS提取物没有显着的遗传毒性潜力。补充信息：在线版本包含补充资料，可在10.1007/s43188-024-00258-9获得。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Toxicological Research TOXICOLOGY-

CiteScore

4.20

自引率

4.30%

发文量

期刊介绍： Toxicological Research is the official journal of the Korean Society of Toxicology. The journal covers all areas of Toxicological Research of chemicals, drugs and environmental agents affecting human and animals, which in turn impact public health. The journal’s mission is to disseminate scientific and technical information on diverse areas of toxicological research. Contributions by toxicologists, molecular biologists, geneticists, biochemists, pharmacologists, clinical researchers and epidemiologists with a global view on public health through toxicological research are welcome. Emphasis will be given to articles providing an understanding of the toxicological mechanisms affecting animal, human and public health. In the case of research articles using natural extracts, detailed information with respect to the origin, extraction method, chemical profiles, and characterization of standard compounds to ensure the reproducible pharmacological activity should be provided.