Hydrogen gas inhalation ameliorates glomerular enlargement after hypoxic-ischemic insult in asphyxiated piglet model.

IF 3.9 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Scientific Reports Pub Date : 2025-01-11 DOI:10.1038/s41598-025-85231-8

Takuma Iwaki, Shinji Nakamura, Takayuki Wakabayashi, Yasuhiro Nakao, Yinmon Htun, Toui Tsuchiya, Tsutomu Mitsuie, Kosuke Koyano, Aya Morimoto, Noriko Fuke, Takayuki Yokota, Sonoko Kondo, Yukihiko Konishi, Takanori Miki, Masaki Ueno, Takashi Iwase, Takashi Kusaka

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Abstract

Acute kidney injury (AKI) has been reported to occur in 30-70% of asphyxiated neonates. Hydrogen (H₂) gas became a major research focus in neonatal medicine after the identification of its robust antioxidative properties. However, the ability of H₂ gas to ameliorate AKI is unknown. We examined histopathological injuries in the piglet renal cortex on day 5 after a hypoxic-ischemic (HI) insult and if H₂ gas can alleviate kidney injuries. Twenty piglets were divided into three groups: no insult (Control, n = 6), HI insult alone (HI, n = 8), and HI insult with H₂ gas ventilation (HI-H₂, 2.1-2.7% for 24 h, n = 6). The total glomerular cell count was significantly higher in the HI group than in the other groups, with no difference between the HI-H₂ and control groups. Proximal tubular lumen narrowing was significantly increased in the HI group versus control, but not in the HI-H₂ group. In this piglet model, glomerular enlargement with an increase in glomerular cell number due to tubular lumen narrowing was observed on day 5 after HI insult. H₂ gas effectively suppressed this glomerular cell increase and tubular lumen narrowing.

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在窒息仔猪模型中吸入氢气可改善缺氧缺血性损伤后的肾小球肿大。

急性肾损伤（AKI）已报道发生在30-70%的窒息新生儿。氢（H2）气体在其强大的抗氧化特性被发现后成为新生儿医学的主要研究热点。然而，氢气改善AKI的能力尚不清楚。我们在缺氧缺血（HI）损伤后第5天检测了仔猪肾皮质的组织病理学损伤，以及H2气体是否可以减轻肾损伤。将20头仔猪分为3组，分别为不侮辱组（对照组，n = 6）、单纯HI侮辱组（HI, n = 8）和HI侮辱加H2气体通气组（HI-H2, 2.1-2.7%，持续24 h， n = 6）。HI组肾小球细胞总数明显高于其他组，HI- h2组与对照组无差异。与对照组相比，HI组近端管腔狭窄明显增加，但HI- h2组没有。在该仔猪模型中，在HI损伤后第5天，观察到肾小球增大，肾小球细胞数量增加，导致小管管腔狭窄。氢气有效地抑制了肾小球细胞的增加和小管腔的狭窄。

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来源期刊

Scientific Reports Natural Science Disciplines-

CiteScore

7.50

自引率

4.30%

发文量

19567

审稿时长

3.9 months

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