Phylogenetic characterization of Bifidobacterium kimbladii sp. nov., a novel species from the honey stomach of the honeybee Apis mellifera

IF 3.3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology Pub Date : 2025-01-01 DOI:10.1016/j.syapm.2025.126579

M. Modesto , D. Scarafile , A. Vásquez , R. Pukall , M. Neumann-Schaal , S. Pascarelli , B. Sgorbati , M. Ancora , C. Cammà , P. Mattarelli , T.C. Olofsson

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引用次数: 0

Abstract

Six novel Bifidobacterium strains H1HS16N^T, Bin2N, Hma3N, H6bp22N, H1HS10N, and H6bp9N, were isolated from the honey stomach of Apis mellifera. Cells are Gram-positive, non-motile, non-sporulating, facultatively anaerobic, and fructose 6-phosphate phosphoketolase-positive. Optimal growth conditions occur at 37 °C in anaerobiosis in MRS medium added with 2 % fructose and 0.1 % L-cysteine. The 16S rRNA gene sequences analysis revealed clustering with Bifidobacterium species found in honeybees. Strains Hma3N, H6bp22N, and H1HS16N^T showed significant similarity to Bifidobacterium polysaccharolyticum JCM 34588^T, with an average similarity of 99.63 %. In contrast, strains Bin2N, H1HS10N, and H6bp9N were closely related to Bifidobacterium apousia JCM 34587^T, with an average similarity of 99.22 %. Moreover, strains Hma3N and H6bp22N exhibited ANI values of 96.65 % and 96.53 % when compared to Bifidobacterium polysaccharolyticum JCM 34588^T, while strains H1HS16N^T, Bin2N, H6bp9N, and H1HS10N revealed ANI values of 94.18 %, 94.33 %, 94.22 %, and 95.50 % respectively when compared to B. apousia JCM 34587^T. dDDH analysis confirmed that strains Hma3N and H6bp22N belong to B. polysaccharolyticum, whereas strains H1HS16N^T, Bin2N, H6bp9N, and H1HS10N represent a novel species. The peptidoglycan of the novel species is of the A4α type (L-Lys-D-Asp). The main cellular fatty acids of the type strain H1HS16N^T are C_16:0, C_14:0, C_19:0 cyclo ω9c, and C_18:1 ω9c. The DNA G + C content of the type strain is 60.8 mol%.

Genome analyses of the strains were also conducted to determine their biosynthesis-related gene clusters, probiotic features, and ecological distribution patterns.

Phenotypic and genotypic characterization show that strain H1HS16N^T is distinct from the type strains of other recognized Bifidobacterium species. Thus, Bifidobacterium kimbladii sp. nov. (H1HS16N^T = DSM 115187^T = CCUG 76695^T) is proposed as a novel Bifidobacterium species.

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蜜蜂蜜胃中的一个新物种--金布拉德双歧杆菌（Bifidobacterium kimbladii sp.

从蜜蜂蜂蜜胃中分离到6株新型双歧杆菌，分别为H1HS16NT、Bin2N、Hma3N、H6bp22N、H1HS10N和H6bp9N。细胞革兰氏阳性，不运动，不产孢，兼性厌氧，果糖6-磷酸磷酸酮酶阳性。在37℃厌氧条件下，在添加2%果糖和0.1% l -半胱氨酸的MRS培养基中生长最佳。16S rRNA基因序列分析显示与蜜蜂中发现的双歧杆菌属聚类。菌株Hma3N、H6bp22N和H1HS16NT与双歧杆菌多糖多糖JCM 34588T具有显著的相似性，平均相似性为99.63%。菌株Bin2N、H1HS10N和H6bp9N与双歧杆菌JCM 34587T亲缘关系较近，平均相似度为99.22%。菌株Hma3N和H6bp22N对双歧杆菌多糖JCM 34588T的ANI值分别为96.65%和96.53%，菌株H1HS16NT、Bin2N、H6bp9N和H1HS10N对双歧杆菌多糖JCM 34587T的ANI值分别为94.18%、94.33%、94.22%和95.50%。dDDH分析证实菌株Hma3N和H6bp22N属于B. polysaccharolyticum，而菌株H1HS16NT、Bin2N、H6bp9N和H1HS10N为新种。新物种的肽聚糖为A4α型（L-Lys-D-Asp）。型菌株H1HS16NT的主要细胞脂肪酸为C16:0、C14:0、C19:0 cyclo ω9c和C18:1 ω9c。该型菌株DNA G + C含量为60.8 mol%。对菌株进行基因组分析，以确定其生物合成相关基因簇、益生菌特征和生态分布模式。表型和基因型分析表明，菌株H1HS16NT不同于其他已知双歧杆菌种的型菌株。因此，提出双歧杆菌kimbladii sp. 11 （H1HS16NT = DSM 115187T = CCUG 76695T）为双歧杆菌新种。

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来源期刊

Systematic and applied microbiology 生物-生物工程与应用微生物

CiteScore

7.50

自引率

5.90%

发文量

审稿时长

22 days

期刊介绍： Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology: