Integration of transcriptomics and metabolomics reveals the mechanism of Glycyrrhizae Radix Et Rhizoma extract inhibiting CCL5 in the treatment of acute pharyngitis.

IF 6.7 1区 医学 Q1 CHEMISTRY, MEDICINAL
Phytomedicine Pub Date : 2025-02-01 Epub Date: 2025-01-03 DOI:10.1016/j.phymed.2024.156360
Xiaoxiao Han, Chengtao Sun, Huizhe Ding, Shengqian Deng, Man Li, Jiayi Lou, Xipeng Song, Guoyin Kai
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引用次数: 0

Abstract

Background: Acute pharyngitis (AP) is a common condition marked by inflammation of the oropharynx, which can lead to severe throat swelling, breathing difficulties, and even suffocation, significantly impacting quality of life. Despite the beneficial anti-inflammatory activity of Glycyrrhizae Radix Et Rhizoma (GRER) and Isoliquiritigenin (ISL), their pharmacological mechanisms against AP remain unclear.

Purpose: This study explores the mechanisms by which GRER treats AP, utilizing both transcriptomics and metabolomics approaches.

Methods: We identified the chemical components of GRER and those that enter the bloodstream using UPLC-MS/MS. Based on15 % ammonia-induced AP model, this study integrates transcriptomics and metabolomics to investigate the mechanism of GRER and ISL in the AP treatment.

Results: The results indicated that GRER has significantly protective and anti-inflammatory effects against AP. Our analysis identified 144 components of GRER in vitro and 17 components in vivo. Network pharmacology and quantitative analysis highlighted ISL as a key active ingredient responsible for GRER's anti-AP effects. Transcriptomics and metabolomics results indicate that GRER and its active ingredient ISL exert therapeutic effects on AP by inhibiting the expression of CCL5 in pharyngeal tissue, thereby downregulating the levels of pro-inflammatory metabolites malic acid and fumaric acid in the tricarboxylic acid (TCA) cycle pathway.

Conclusion: The data in this article demonstrated that GRER and ISL has significantly anti-inflammatory effects and protective effects for AP by regulating CCL5 expression to reduce the levels of pro-inflammatory metabolites within TCA cycle pathway. It provides a scientific basis for prevention and treatment of AP.

整合转录组学和代谢组学揭示甘草提取物抑制CCL5治疗急性咽炎的机制
背景:急性咽炎(AP)是一种以口咽部炎症为特征的常见疾病,可导致严重的喉咙肿胀,呼吸困难,甚至窒息,严重影响生活质量。尽管Glycyrrhizae Radix Et Rhizoma (GRER)和Isoliquiritigenin (ISL)具有有益的抗炎活性,但其抗AP的药理机制尚不清楚。目的:本研究利用转录组学和代谢组学方法探讨GRER治疗AP的机制。方法:采用超高效液相色谱-串联质谱(UPLC-MS/MS)技术对其化学成分和进入血液的化学成分进行鉴定。本研究基于15%氨诱导AP模型,结合转录组学和代谢组学研究GRER和ISL在AP治疗中的作用机制。结果:GRER对AP具有明显的保护和抗炎作用,体外鉴定出144种成分,体内鉴定出17种成分。网络药理学和定量分析表明,ISL是GRER抗ap作用的关键活性成分。转录组学和代谢组学结果表明,GRER及其活性成分ISL通过抑制咽部组织中CCL5的表达,从而下调三羧酸(TCA)循环通路中促炎代谢物苹果酸和富马酸的水平,对AP发挥治疗作用。结论:本文数据表明,GRER和ISL通过调节CCL5的表达,降低TCA循环通路中促炎代谢物的水平,对AP具有显著的抗炎和保护作用。为AP的防治提供了科学依据。
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来源期刊
Phytomedicine
Phytomedicine 医学-药学
CiteScore
10.30
自引率
5.10%
发文量
670
审稿时长
91 days
期刊介绍: Phytomedicine is a therapy-oriented journal that publishes innovative studies on the efficacy, safety, quality, and mechanisms of action of specified plant extracts, phytopharmaceuticals, and their isolated constituents. This includes clinical, pharmacological, pharmacokinetic, and toxicological studies of herbal medicinal products, preparations, and purified compounds with defined and consistent quality, ensuring reproducible pharmacological activity. Founded in 1994, Phytomedicine aims to focus and stimulate research in this field and establish internationally accepted scientific standards for pharmacological studies, proof of clinical efficacy, and safety of phytomedicines.
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