WD40 proteins PaTTG1 interact with both bHLH and MYB to regulate trichome formation and anthocyanin biosynthesis in Platanus acerifolia

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-01-09 DOI:10.1016/j.plantsci.2025.112385

Qi Mengxuan, Tian Xinyue, Chen Yuqing, Lu Yongkang, Zhang Yanping

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引用次数: 0

Abstract

Trichome development and anthocyanin accumulation are regulated by a complex regulatory network, the MBW complexe consists of MYB, bHLH, and WD40 transcription factors. In this study, two sequences, named PaTTG1.1, and PaTTG1.2, were cloned and functionally characterized from Platanus acerifolia. Quantitative real-time PCR results showed that PaTTG1 genes were expressed in the trichomes and red leaves. Overexpression of PaTTG1.1 and PaTTG1.2 genes in Arabidopsis ttg1 mutants restored the phenotypes of ttg1 mutants that were glabrous and lacked purple anthocyanins in hypocotyls and seeds. In transgenic plants, the expression levels of the trichome regulation-related genes AtCPC, AtTRY, AtETC1, AtMYB23, and AtGL2, as well as early and late biosynthetic genes related to anthocyanin biosynthesis, were significantly upregulated. The results of the yeast two-hybrid showed that PaTTG1.1 and PaTTG1.2 proteins could physically interact with both bHLH and R2R3-MYB transcription factors from Arabidopsis and P. Acerifolia. Taken together, the results presented in this study suggest that the two PaTTG1 genes share similar functions in the regulation of trichomes and anthocyanins. However, there may be some differences in their regulatory mechanisms.

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WD40蛋白PaTTG1与bHLH和MYB相互作用，调节毛囊形成和花青素的生物合成。

毛状体发育和花青素积累受一个复杂的调控网络调控，MBW复合物由MYB、bHLH和WD40转录因子组成。本研究克隆了两个序列，命名为PaTTG1.1和PaTTG1.2，并对其进行了功能鉴定。实时荧光定量PCR结果显示，PaTTG1基因在毛状体和红叶中均有表达。在拟南芥ttg1突变体中过表达PaTTG1.1和PaTTG1.2基因，恢复了ttg1突变体下胚轴和种子中无毛且缺乏紫色花青素的表型。在转基因植株中，毛状体调控相关基因AtCPC、AtTRY、AtETC1、AtMYB23、AtGL2以及与花青素生物合成相关的早期和晚期生物合成基因的表达水平均显著上调。酵母双杂交结果表明，PaTTG1.1和PaTTG1.2蛋白可与拟南芥和荆芥的bHLH和R2R3-MYB转录因子发生物理相互作用。综上所述，本研究的结果表明，在不同物种中，两个PaTTG1基因在调节毛状体和花青素方面具有相似的功能。然而，它们的监管机制可能存在一些差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.