Rice THIN CULM 4 (TC4) modulates culm strength by regulating morphology, structure, and development

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-01-10 DOI:10.1016/j.plantsci.2024.112375

Ran Zhou , Chenbo Huang , Xiaoxia Wen , Zhihao Sun , Wei Dong , Yuyu Chen , Nuan Huang , Han Zhang , Haihan Su , Yanhui Li , Zequn Peng , Yingxin Zhang , Liyong Cao , Shihua Cheng , Xiaodeng Zhan , Lianping Sun , Daibo Chen

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引用次数: 0

Abstract

Culm strength is crucial for rice growth, nutrition transportation, and structural resilience, which are essential for lodging resistance and stable production. In this study, we identified a rice thin culm mutant tc4, characterized by thinner culms and thicker cavity walls, resulting in weakened culm mechanical strength. Using map-based cloning, the candidate gene was isolated, and complementation and CRISPR/Cas9 experiments confirmed that a single nucleotide substitution in TC4 is responsible for the thin and brittle culm phenotype. TC4, a homolog of the FLORICAULA/LEAFY gene, localizes to the nucleus and cytoplasm. Further research revealed that TC4 regulates culm development by influencing plant hormones and sugar transport. This research not only advances our understanding of rice culm regulation, but also provides valuable insights for breeding lodging-resistant rice varieties.

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水稻 THIN CULM 4 (TC4) 通过调节形态、结构和发育来调节茎秆强度。

茎秆强度对水稻生长、营养转运和结构抗逆性至关重要，对水稻抗倒伏和稳定生产至关重要。在这项研究中，我们鉴定了一个水稻薄秆突变体tc4，其特征是茎变薄，空腔壁变厚，导致茎的机械强度减弱。利用图谱克隆技术分离出候选基因，并进行了互补和CRISPR/Cas9实验，证实TC4中的单核苷酸替换是导致茎薄脆表型的原因。TC4是FLORICAULA/LEAFY基因的同源物，定位于细胞核和细胞质中。进一步研究发现，TC4通过影响植物激素和糖的转运来调控茎的发育。该研究不仅提高了我们对水稻茎秆调控的认识，而且为水稻抗倒伏品种的选育提供了有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.