Technical Advances Drive the Molecular Understanding of Effectors from Wheat and Barley Powdery Mildew Fungi.

Abstract

Pathogens manipulate host physiology through the secretion of virulence factors (effectors) to invade and proliferate on the host. The molecular functions of effectors inside plant hosts have been of interest in the field of molecular plant-microbe interactions. Obligate biotrophic pathogens, such as rusts and powdery mildews, cannot proliferate outside of plant hosts. In addition to the inhibition of the plant's immune components, these pathogens are under particular pressure to extract nutrients efficiently from the host. Understanding the molecular basis of infections mediated by obligate biotrophic pathogens is significant because of their impact in modern agriculture. In addition, powdery mildews serve as excellent models for obligate biotrophic cereal pathogens. Here, we summarize the current knowledge on the effectorome of the barley and wheat powdery mildews and putative molecular virulence functions of effectors. We emphasize the availability of comprehensive genomic, transcriptomic, and proteomic resources and discuss the methodological approaches used for identifying candidate effectors, assessing effector virulence traits, and identifying effector targets in the host. We highlight established and more recently employed methodologies, discuss limitations, and suggest additional strategies. We identify open questions and discuss how addressing them with currently available resources will enhance our understanding of Blumeria candidates for secretor effector proteins. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.