Effect of reduced placental expression of zinc transporters on selective intrauterine growth restriction in monochorionic diamniotic twin pregnancies

IF 4.8 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Nutritional Biochemistry Pub Date : 2025-01-10 DOI:10.1016/j.jnutbio.2025.109840

Tingting Xu , Yao Kong , Qing Hu , Hua Liao , Chunyan Deng , Xiaodong Wang , Haiyan Yu

{"title":"Effect of reduced placental expression of zinc transporters on selective intrauterine growth restriction in monochorionic diamniotic twin pregnancies","authors":"Tingting Xu , Yao Kong , Qing Hu , Hua Liao , Chunyan Deng , Xiaodong Wang , Haiyan Yu","doi":"10.1016/j.jnutbio.2025.109840","DOIUrl":null,"url":null,"abstract":"<div><div>Zinc is an essential trace element. The regulatory mechanism of zinc and its transporters in fetal growth in monochorionic diamniotic (MCDA) twins with selective intrauterine growth restriction (MCDA-sIUGR) is unclear. A total of 45 MCDA twins were divided into two groups, MCDA (n=37) and MCDA-sIUGR (n=8), to investigate their possible effects on fetal growth. Maternal and fetal serum zinc levels were measured using a microassay. Immunohistochemistry and western blotting were performed to quantify the expression and localization of zinc transporters. Correlation scatter plots and matrices were used to test the correlation between maternal and cord serum zinc levels, placental zinc transporters, and fetal growth. There was a significant difference in fetal birth weight and placental weight among MCDA TH (higher birth weight of twin), MCDA TL (lower birth weight of twin), MCDA-sIUGR TH, and MCDA-sIUGR TL groups (P < .05), among which the MCDA-sIUGR TL group was found to be significantly lower than the other three groups (P < .05). Placental zinc transporters, including Zip 2, metal regulatory transcription factor 1 (MTF 1), and ZnT1, were significantly decreased in the MCDA-sIUGR TL group compared to those in the other three groups (P < .05). Furthermore, we observed varying degrees of correlation between fetal weight and maternal/fetal serum zinc levels and placental zinc transporters. Our study indicates that placental zinc transporters are impaired in MCDA-sIUGR, particularly in lightweight twins. Therefore, we hypothesized that the reduced placental expression of zinc transporters may affect fetal growth in MCDA twins.</div></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":"137 ","pages":"Article 109840"},"PeriodicalIF":4.8000,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Nutritional Biochemistry","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0955286325000038","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Zinc is an essential trace element. The regulatory mechanism of zinc and its transporters in fetal growth in monochorionic diamniotic (MCDA) twins with selective intrauterine growth restriction (MCDA-sIUGR) is unclear. A total of 45 MCDA twins were divided into two groups, MCDA (n=37) and MCDA-sIUGR (n=8), to investigate their possible effects on fetal growth. Maternal and fetal serum zinc levels were measured using a microassay. Immunohistochemistry and western blotting were performed to quantify the expression and localization of zinc transporters. Correlation scatter plots and matrices were used to test the correlation between maternal and cord serum zinc levels, placental zinc transporters, and fetal growth. There was a significant difference in fetal birth weight and placental weight among MCDA T_H (higher birth weight of twin), MCDA T_L (lower birth weight of twin), MCDA-sIUGR T_H, and MCDA-sIUGR T_L groups (P < .05), among which the MCDA-sIUGR T_L group was found to be significantly lower than the other three groups (P < .05). Placental zinc transporters, including Zip 2, metal regulatory transcription factor 1 (MTF 1), and ZnT1, were significantly decreased in the MCDA-sIUGR T_L group compared to those in the other three groups (P < .05). Furthermore, we observed varying degrees of correlation between fetal weight and maternal/fetal serum zinc levels and placental zinc transporters. Our study indicates that placental zinc transporters are impaired in MCDA-sIUGR, particularly in lightweight twins. Therefore, we hypothesized that the reduced placental expression of zinc transporters may affect fetal growth in MCDA twins.

Abstract Image

查看原文本刊更多论文

胎盘锌转运蛋白表达降低对双羊膜双胎妊娠选择性宫内生长限制的影响。

锌是人体必需的微量元素。锌及其转运体在选择性宫内生长受限（MCDA- siugr）单绒毛膜双羊膜双胞胎（MCDA- siugr）胎儿生长中的调节机制尚不清楚。将45例MCDA双胞胎分为MCDA组（n=37）和MCDA- siugr组（n=8），探讨其对胎儿生长的可能影响。采用微量法测定孕妇和胎儿血清锌水平。免疫组织化学和免疫印迹法定量测定锌转运蛋白的表达和定位。使用相关散点图和矩阵来检验母体和脐带血清锌水平、胎盘锌转运蛋白和胎儿生长之间的相关性。MCDA TH组（双胎高出生体重）、MCDA TL组（双胎低出生体重）、MCDA- siugr TH组和MCDA- siugr TL组的胎儿出生体重和胎盘重量差异有统计学意义(pL组显著低于其他三组（pL组与其他三组比较差异有统计学意义）

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Nutritional Biochemistry 医学-生化与分子生物学

CiteScore

9.50

自引率

3.60%

发文量

237

审稿时长

68 days

期刊介绍： Devoted to advancements in nutritional sciences, The Journal of Nutritional Biochemistry presents experimental nutrition research as it relates to: biochemistry, molecular biology, toxicology, or physiology. Rigorous reviews by an international editorial board of distinguished scientists ensure publication of the most current and key research being conducted in nutrition at the cellular, animal and human level. In addition to its monthly features of critical reviews and research articles, The Journal of Nutritional Biochemistry also periodically publishes emerging issues, experimental methods, and other types of articles.