Exposure to a choline-deficient diet during pregnancy and lactation alters the liver transcriptome profile in offspring of dams with fatty liver.

IF 2.9 Q3 NUTRITION & DIETETICS

Clinical nutrition ESPEN Pub Date : 2025-01-10 DOI:10.1016/j.clnesp.2025.01.028

Joanna Mikołajczyk-Stecyna, Ewelina Zuk, Agata Chmurzynska, Malgorzata Blatkiewicz, Karol Jopek, Marcin Rucinski

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引用次数: 0

Abstract

Background & aims: The developmental origin of health and disease hypothesis shows that early adverse exposures can have lifelong health effects. Thus, the aim of this study was to analyze the impact of choline intake during pregnancy and/or lactation on gene expression profiles in the liver of 24-day-old male rat offspring from dams with non-alcoholic fatty liver disease (NAFLD).

Methods: Phenotypic characteristic, histological examination and global transcriptome pattern of liver tissue specimens obtained from offspring of dams suffering from fatty liver, provided with proper choline intake during pregnancy and lactation (NN), fed a choline-deficient diet during both periods (DD), deprived of choline only during pregnancy (DN), or only during lactation (ND), was performed. The global gene expression profile was analyzed by using microarray approach (Affymetrix® Rat Gene 2.1 ST Array Strip). The relative expression of selected genes was validated by real-time polymerase chain reaction (qPCR).

Results: The histological examination of rat liver sections indicated alternations typical for fatty liver in all analyzed groups with increased progression among groups deprived of choline. Choline deficiency in the maternal diet was associated with changes in body mass and composition but not with biochemical marker levels, except for the high density lipoprotein fraction of cholesterol (HDL). Enhanced expression of genes involved in oxidative stress, cell proliferation, activation of catabolic processes related to hepatocyte dysfunction and cell membrane composition were simultaneously observed in all choline-deficient groups.

Conclusions: An adequate amount of choline in the diet of a mother with fatty liver during pregnancy and/or lactation can regulate gene expression in the offspring's liver and contribute to a milder stage of the disease in the progeny. Moreover, proper choline supply during the postpartum period is as crucial as during the prenatal period.

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妊娠期和哺乳期胆碱缺乏的饮食会改变脂肪肝母鼠后代的肝脏转录组谱。

背景与目的：健康与疾病的发育起源假说表明，早期不良接触可能对健康产生终身影响。因此，本研究的目的是分析妊娠和/或哺乳期胆碱摄入量对24日龄非酒精性脂肪性肝病（NAFLD）雄性大鼠后代肝脏基因表达谱的影响。方法：对脂肪肝母鼠的后代肝脏组织标本进行表型特征、组织学检查和整体转录组分析，分别在妊娠和哺乳期（NN）给予适当的胆碱摄入，在两期（DD）均饲喂缺乏胆碱的饮食，仅在妊娠期（DN）或仅在哺乳期（ND）剥夺胆碱。采用微阵列方法（Affymetrix®大鼠基因2.1 ST阵列条带）分析全局基因表达谱。所选基因的相对表达量通过实时聚合酶链反应（qPCR）进行验证。结果：大鼠肝脏切片的组织学检查显示，在所有分析组中，脂肪肝的改变都是典型的，在胆碱剥夺组中，进展加剧。母亲饮食中胆碱缺乏与体重和组成的变化有关，但与生化标志物水平无关，除了胆固醇的高密度脂蛋白部分（HDL）。在所有胆碱缺乏组中，参与氧化应激、细胞增殖、与肝细胞功能障碍和细胞膜组成相关的分解代谢过程的激活的基因表达同时增强。结论：患有脂肪肝的母亲在妊娠和/或哺乳期饮食中摄入足量的胆碱可以调节后代肝脏中的基因表达，并有助于后代病情较轻。此外，产后适当的胆碱供应与产前一样重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical nutrition ESPEN NUTRITION & DIETETICS-

CiteScore

4.90

自引率

3.30%

发文量

512

期刊介绍： Clinical Nutrition ESPEN is an electronic-only journal and is an official publication of the European Society for Clinical Nutrition and Metabolism (ESPEN). Nutrition and nutritional care have gained wide clinical and scientific interest during the past decades. The increasing knowledge of metabolic disturbances and nutritional assessment in chronic and acute diseases has stimulated rapid advances in design, development and clinical application of nutritional support. The aims of ESPEN are to encourage the rapid diffusion of knowledge and its application in the field of clinical nutrition and metabolism. Published bimonthly, Clinical Nutrition ESPEN focuses on publishing articles on the relationship between nutrition and disease in the setting of basic science and clinical practice. Clinical Nutrition ESPEN is available to all members of ESPEN and to all subscribers of Clinical Nutrition.