{"title":"Myceliophthora thermophila as promising fungal cell factories for industrial bioproduction: From rational design to industrial applications.","authors":"Zhengshan Luo, Yue Gao, Xupeng Guo, Yilin Chen, Yijian Rao","doi":"10.1016/j.biortech.2025.132051","DOIUrl":null,"url":null,"abstract":"<p><p>Myceliophthora thermophila stands out as a prominent fungal cell factory, garnering growing interest due to its distinctive traits advantageous. Currently, M. thermophila has been developed as an efficient cell factory, producing a variety of products from various raw materials. In this review, we firstly discuss the potential advantages of M. thermophila as a platform for metabolic engineering and industrial applications, with special emphasis on its physiological characteristics, the development of genetic modification techniques and tools, gene expression and regulation strategies. Then, the latest progress in industrial application of M. thermophila as microbial cell factory was systematically summarized, including biochemical synthesis platform, enzyme expression platform, antibody protein and vaccine production platform, bio-organic fertilizer production platform, and efficient enzyme element library. Finally, the current challenges of M. thermophila as a cell factory and its corresponding strategies are proposed, aiming to achieve green biomanufacturing of multiple products with higher efficiency.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132051"},"PeriodicalIF":9.7000,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioresource Technology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1016/j.biortech.2025.132051","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURAL ENGINEERING","Score":null,"Total":0}
引用次数: 0
Abstract
Myceliophthora thermophila stands out as a prominent fungal cell factory, garnering growing interest due to its distinctive traits advantageous. Currently, M. thermophila has been developed as an efficient cell factory, producing a variety of products from various raw materials. In this review, we firstly discuss the potential advantages of M. thermophila as a platform for metabolic engineering and industrial applications, with special emphasis on its physiological characteristics, the development of genetic modification techniques and tools, gene expression and regulation strategies. Then, the latest progress in industrial application of M. thermophila as microbial cell factory was systematically summarized, including biochemical synthesis platform, enzyme expression platform, antibody protein and vaccine production platform, bio-organic fertilizer production platform, and efficient enzyme element library. Finally, the current challenges of M. thermophila as a cell factory and its corresponding strategies are proposed, aiming to achieve green biomanufacturing of multiple products with higher efficiency.
期刊介绍:
Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies.
Topics include:
• Biofuels: liquid and gaseous biofuels production, modeling and economics
• Bioprocesses and bioproducts: biocatalysis and fermentations
• Biomass and feedstocks utilization: bioconversion of agro-industrial residues
• Environmental protection: biological waste treatment
• Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.