VvPIP1;1 plays a role in grape berry cracking by regulating water uptake

IF 5.7 1区 农林科学 Q1 HORTICULTURE
Jun Yu, Jia Li, Zhangfen Hong, Qing Zeng, Yizheng Fu, Rouxue Deng, Ke Xu, Zhenyu Huang, Mingtao Zhu
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Abstract

Fruit cracking is a persistent challenge for table grape growing. To investigate the mechanism of this disorder, a comprehensive two-year investigation was conducted to assess the fruit cracking percentage of 15 table grape (Vitis vinifera L.) varieties. Based on the findings, the cracking-susceptible variety ‘Xiangfei’ and the cracking-resistant variety ‘Zuijinxiang’ were selected for further study. Fruit growth curves for ‘Zuijinxiang’ and ‘Xiangfei’ were plotted based on fruit diameter and total soluble solids content, revealing that both varieties exhibited typical double-sigmoidal patterns that were highly similar. The period between 48 and 53 days after full bloom (DAFB) was identified as the critical phase for fruit cracking incidence. Furthermore, during the fruit cracking period, ‘Xiangfei’ fruit exhibited significantly higher water content and mesocarp cell area compared with those of ‘Zuijinxiang’. Applying aquaporin inhibitors (nano-silver) to ‘Xiangfei’ berries reduced fruit water uptake and cracking percentage, whereas applying aquaporin activators (forskolin) to ‘Zuijinxiang’ berries increased fruit water uptake and cracking percentage. Additionally, expression analysis of six genes associated with plasma membrane intrinsic proteins (PIPs) synthesis (VvPIP1;1, VvPIP1;2, VvPIP1;3, VvPIP2;1, VvPIP2;2, and VvPIP2;3) revealed that only the expression level of VvPIP1;1 was higher in ‘Zuijinxiang’ than in ‘Xiangfei’ during the fruit cracking period, whereas the expression levels of the other genes exhibited no significant difference between the two varieties. Transgenic overexpression of VvPIP1;1 in tomato resulted in increased fruit water content, enlarged mesocarp cell size, and enhanced fruit cracking percentage. These findings indicate that VvPIP1;1 plays a pivotal role in controlling grape berry cracking.
vppip1;1通过调节水分吸收在葡萄果实开裂中起作用
水果开裂是食用葡萄种植的一个持久的挑战。为了探讨这种病害的发生机制,对15个鲜食葡萄品种进行了为期两年的果实开裂率调查。在此基础上,选择易裂品种“香飞”和抗裂品种“醉金香”进行进一步研究。根据果实直径和总可溶性固形物含量绘制了‘醉金香’和‘香飞’的果实生长曲线,结果表明,这两个品种都表现出典型的双s型曲线,两者高度相似。结果表明,开花后48 ~ 53 d是果实开裂发生的关键时期。在果实开裂期,‘香飞’果实的含水量和中果皮细胞面积显著高于‘醉金香’。在“香飞”浆果上施用水通道蛋白抑制剂(纳米银)降低了果实的吸水率和开裂率,而在“醉金香”浆果上施用水通道蛋白活化剂(forskolin)提高了果实的吸水率和开裂率。此外,对6个与质膜内在蛋白(PIPs)合成相关的基因(VvPIP1;1, VvPIP1;2, VvPIP1;3, VvPIP2;1, VvPIP2;2, VvPIP2;3)的表达分析显示,在果实破裂期,只有VvPIP1;1在“醉金香”中的表达水平高于“湘非”,而其他基因的表达水平在两个品种之间无显著差异。在番茄中转基因过表达VvPIP1;1导致果实含水量增加,中果皮细胞大小增大,果实开裂率提高。这些发现表明,VvPIP1;1在控制葡萄果实开裂中起着关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Horticultural Plant Journal
Horticultural Plant Journal Environmental Science-Ecology
CiteScore
9.60
自引率
14.00%
发文量
293
审稿时长
33 weeks
期刊介绍: Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.
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