Capture primed pluripotency in guinea pig.

IF 5.9 2区医学 Q1 CELL & TISSUE ENGINEERING

Stem Cell Reports Pub Date : 2024-12-27 DOI:10.1016/j.stemcr.2024.102388

Jing Guo, Runxia Lin, Jinpeng Liu, Rongrong Liu, Shuyan Chen, Zhen Zhang, Yongzheng Yang, Haiyun Wang, Luqin Wang, Shengyong Yu, Chunhua Zhou, Lizhan Xiao, Rongping Luo, Jinjin Yu, Lihua Zeng, Xiaoli Zhang, Yusha Li, Haokaifeng Wu, Tao Wang, Yi Li, Manish Kumar, Ping Zhu, Jing Liu

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引用次数: 0

Abstract

Guinea pigs are valuable models for human disease research, yet the lack of established pluripotent stem cell lines has limited their utility. In this study, we isolate and characterize guinea pig epiblast stem cells (gpEpiSCs) from post-implantation embryos. These cells differentiate into the three germ layers, maintain normal karyotypes, and rely on FGF2 and ACTIVIN A signaling for self-renewal and pluripotency. Wingless/Integrated (WNT) signaling inhibition is also essential for their maintenance. GpEpiSCs express key pluripotency markers (OCT4, SOX2, NANOG) and share transcriptional similarities with human and mouse primed stem cells. While many genes are conserved between guinea pig and human primed stem cells, transcriptional analysis also reveals species-specific differences in pluripotency-related pathways. Epigenetic analysis highlights bivalent gene regulation, underscoring their developmental potential. This work demonstrates both the evolutionary conservation and divergence of primed pluripotent stem cells, providing a new tool for biomedical research and enhancing guinea pigs' utility in studying human diseases.

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豚鼠捕获引物多能性。

豚鼠是人类疾病研究的宝贵模型，但缺乏成熟的多能干细胞系限制了它们的应用。在这项研究中，我们从植入后胚胎中分离并表征了豚鼠外胚层干细胞（gpEpiSCs）。这些细胞分化成三个胚层，维持正常的核型，并依靠FGF2和ACTIVIN A信号进行自我更新和多能性。无翼/集成（WNT）信号抑制对其维持也是必不可少的。GpEpiSCs表达关键的多能性标记（OCT4, SOX2， NANOG），并且与人和小鼠干细胞具有转录相似性。虽然许多基因在豚鼠和人类干细胞之间是保守的，转录分析也揭示了多能性相关途径的物种特异性差异。表观遗传学分析强调了二价基因调控，强调了它们的发育潜力。这项工作证明了引物多能干细胞的进化保守性和分化性，为生物医学研究提供了新的工具，并提高了豚鼠在人类疾病研究中的实用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Stem Cell Reports CELL & TISSUE ENGINEERING-CELL BIOLOGY

CiteScore

10.50

自引率

1.70%

发文量

200

审稿时长

28 weeks

期刊介绍： Stem Cell Reports publishes high-quality, peer-reviewed research presenting conceptual or practical advances across the breadth of stem cell research and its applications to medicine. Our particular focus on shorter, single-point articles, timely publication, strong editorial decision-making and scientific input by leaders in the field and a "scoop protection" mechanism are reasons to submit your best papers.