Cu-tolerant Klebsiella variicola SRB-4 increased the nanoparticle (NP) stress resilience in garden peas (Pisum sativum L.) raised in soil polluted with varying doses of copper oxide (CuO)-NP.

IF 4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

World journal of microbiology & biotechnology Pub Date : 2025-01-11 DOI:10.1007/s11274-024-04239-w

Mohammad Danish, Mohammad Shahid, Zaryab Shafi, Mohammad Abul Farah, Khalid Mashay Al-Anazi

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引用次数: 0

Abstract

Utilizing metal/nanoparticle (NP)- tolerant plant growth-promoting rhizobacteria (PGPR) is a sustainable and eco-friendly approach for remediation of NP-induced phytotoxicity. Here, Pisum sativum (L.) plants co-cultivated with different CuO-NP concentrations exhibited reduced growth, leaf pigments, yield attributes, and increased oxidative stress levels. Cu-tolerant (800 µM) Klebsiella variicola strain SRB-4 (Accession no. OR715781.1) recovered from metal-contaminated soils produced various PGP traits, including IAA, EPS, siderophore, HCN, ammonia, and solubilized insoluble P. The PGP substances were marginally increased with increasing CuO-NP concentrations. When applied, Cu-tolerant SRB-4 strain increased root length (18%), root biomass (15.3%), total chlorophyll (29%), carotenoids (30%), root N (21%), root P (23%), total soluble protein (20%) nodule number (32%), nodule biomass (39%) and leghaemoglobin content (18%) in 50 µM CuO-NP-exposed peas. Furthermore, proline, malondialdehyde (MDA), superoxide radical, hydrogen peroxide (H₂O₂) content, and membrane injury in K. variicola-inoculated and 50 µM CuO-NP-treated plants were maximally and significantly (p ≤ 0.05) reduced by 70.6, 26.8, 60.8, and 71.6%, respectively, over uninoculated but treated with similar NP doses. Moreover, K. variicola inoculation caused a significant (p ≤ 0.05) decline in Cu uptake in roots (71%), shoots (65.5%), and grains (76.4%) of peas grown in soil contaminated with 50 µM CuO-NP. The multivariate i.e. heat map and pearson correlation analyses between the NP-treated and PGPR inoculated parameters revealed a significant and strong positive corelation. The NP-tolerant indigenous beneficial K. variicola could be applied as an alternative to enhance the production of P. sativum cultivated in nano-polluted soil systems. Additionally, more investigation is required to ascertain the seed/soil inoculation effect of K. variicola SRB-4 on soil biological activities and different crops under various experimental setups.

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在不同剂量氧化铜-NP污染的土壤中，耐铜的水痘克雷伯菌SRB-4提高了豌豆的纳米颗粒（NP）抗逆性。

利用耐金属/纳米颗粒（NP）植物促生长根瘤菌（PGPR）修复NP诱导的植物毒性是一种可持续和环保的方法。在不同CuO-NP浓度下共培养的Pisum sativum （L.）植株表现出生长、叶色素、产量属性降低和氧化胁迫水平升高的现象。耐铜（800µM）水痘克雷伯菌SRB-4 (Accession no.；从金属污染土壤中回收的OR715781.1)产生了多种PGP特征，包括IAA、EPS、铁载体、HCN、氨和可溶性不溶性p， PGP物质随CuO-NP浓度的增加而略有增加。施用耐铜菌株SRB-4后，50µM cuo - np处理的豌豆根长（18%）、根生物量（15.3%）、总叶绿素（29%）、类胡萝卜素（30%）、根氮（21%）、根磷（23%）、总可溶性蛋白（20%）、根瘤数（32%）、根瘤生物量（39%）和豆血红蛋白含量（18%）均增加。与未接种相同NP剂量的植株相比，接种50µM cuo -NP的植株脯氨酸、丙二醛（MDA）、超氧自由基、过氧化氢（H2O2）含量和膜损伤分别显著降低70.6、26.8、60.8和71.6% （p≤0.05）。在50µM CuO-NP污染的土壤中，接种天花小分枝杆菌后，豌豆根系、茎部和籽粒对铜的吸收量均显著下降（p≤0.05），分别为71%、65.5%和76.4%。np处理与PGPR接种参数之间的多变量热图分析和pearson相关分析显示了显著且强的正相关。耐np的本土有益品种variicola可作为在纳米污染土壤系统中提高sativum产量的替代品种。此外，在不同的试验条件下，还需要进一步的研究来确定SRB-4对土壤生物活性和不同作物的接种效果。

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来源期刊

World journal of microbiology & biotechnology 工程技术-生物工程与应用微生物

CiteScore

6.30

自引率

2.40%

发文量

257

审稿时长

2.5 months

期刊介绍： World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology. Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions. Some topics are not within the scope of the Journal. Please do not submit your manuscript if it falls into one of the following categories: · Virology · Simple isolation of microbes from local sources · Simple descriptions of an environment or reports on a procedure · Veterinary, agricultural and clinical topics in which the main focus is not on a microorganism · Data reporting on host response to microbes · Optimization of a procedure · Description of the biological effects of not fully identified compounds or undefined extracts of natural origin · Data on not fully purified enzymes or procedures in which they are applied All articles published in the Journal are independently refereed.