Transcriptomics and Proteomics Analysis of the Liver of RAD52 Knockout Mice.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2025-01-02 DOI:10.3390/ijms26010339

Yingjie Song, Lan Yang, Yao Han, Wenjun Li, Tong Wei, Yamin Gao, Qiang Hu, Hao Li, Yansong Sun

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引用次数: 0

Abstract

RAD52 plays crucial roles in several aspects of mammalian cells, including DNA double-strand breaks repair, viral infection, cancer development, and antibody class switching. To comprehensively elucidate the role of RAD52 in maintaining genome stability and uncover additional functions of RAD52 in mammals, we performed the transcriptomics and proteomics analysis of the liver of RAD52 knockout mice. Transcriptomics analysis reveals overexpression of mitochondrial genes in the liver of RAD52 knockout (RAD52KO) mice. Proteomics analysis of RAD52KO mice shows that damage recognition proteins Cul4b and Rad23a in the process of nucleotide excision repair pathway are overexpressed. Furthermore, gene ontology and KEGG enrichment analysis (accessed on 20 November 2024) from integrated omics shows that differentially expressed genes are significantly enriched in pathways related to mitochondrial oxidative phosphorylation and nucleotide metabolism in the liver of RAD52KO mice. In addition, mRNA and protein levels of Bhmt1b are elevated in the liver of RAD52KO mice. Taken together, this study provides valuable insights into the function and mechanism of RAD52.

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RAD52基因敲除小鼠肝脏转录组学和蛋白质组学分析。

RAD52 在哺乳动物细胞的多个方面发挥着关键作用，包括 DNA 双链断裂修复、病毒感染、癌症发展和抗体类别转换。为了全面阐明 RAD52 在维持基因组稳定性中的作用，并揭示 RAD52 在哺乳动物中的其他功能，我们对 RAD52 基因敲除小鼠的肝脏进行了转录组学和蛋白质组学分析。转录组学分析显示，RAD52基因敲除（RAD52KO）小鼠肝脏中的线粒体基因过度表达。RAD52KO 小鼠的蛋白质组学分析表明，核苷酸切除修复途径过程中的损伤识别蛋白 Cul4b 和 Rad23a 表达过高。此外，基因本体和 KEGG 富集分析（2024 年 11 月 20 日访问）显示，在 RAD52KO 小鼠肝脏中，与线粒体氧化磷酸化和核苷酸代谢相关的通路中显著富集了差异表达基因。此外，RAD52KO 小鼠肝脏中 Bhmt1b 的 mRNA 和蛋白水平均升高。总之，这项研究为了解 RAD52 的功能和机制提供了宝贵的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Molecular Sciences 化学-化学综合

自引率

10.70%

发文量

13472

审稿时长

1.7 months

期刊介绍： The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).