Molecular and serological investigation of Brucella species in kennel and farm dogs in Iran

IF 2.1 3区医学 Q2 PARASITOLOGY

Acta tropica Pub Date : 2025-02-01 DOI:10.1016/j.actatropica.2025.107521

Baharak Akhtardanesh , Elham Mohammadi , Soheil Sadr , Asma Askari , Zeinab Manzari Tavakoli , Rozhin Ahmadi , Shakiba Nazemian , Hossein Rashidi , Morteza Aghamiri , Mehdi Golchin , Masoud Imani

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引用次数: 0

Abstract

Introduction

Brucellosis is still a significant emerging threat to public health, as it can infect humans, wild, domestic animals, and livestock. Hence, the current study aims to determine the frequency of canine brucellosis (CB), its relationship with clinical findings and reproductive disorders in kennel and farm dogs, and its importance on public health.

Materials and methods

From January 2022 to December 2023, a total of 150 blood samples were taken from 100 adult dogs in breeding kennels and 50 shepherd dogs in breeding farms in Kerman, Iran. Rose Bengal test (RBT) and Wright tests were used for the primary screening of Brucella abortus (B. abortus) and Brucella melitensis (B. melitensis) species, and conventional IS711-based PCR and real-time polymerase chain reaction (RT-PCR) were performed to determine the Brucella genus and species in all samples.

Findings

In kennel dogs, 34 % (34/100) had a history of breeding disorders, and 30 % (30/100) were RBT-positive. Moreover, B. canis and B. abortus species were detected in kennel dogs. Among farm dogs, 16 % (8/50) had a history of breeding disorders, and 28 % (14/50) were RBT positive. Additionally, B. canis, B. abortus, and B. melitensis species were detected in farm dogs. Feeding with unpasteurized milk was significantly related to a positive RBT in kennel dogs (p = 0.009), and there was a significant correlation between breeding disorders and seropositivity in kennel (p = 0.045) and farm dogs (p = 0.03).

Conclusion

The current study represents a significant advancement in understanding CB in Iran by the first molecular detection of B. canis, revealing B. melitensis and B. abortus as important pathogens in kennel and farm dogs and highlighting the public health significance of disease in Iran.

查看原文本刊更多论文

伊朗犬舍和农场犬布鲁氏菌的分子和血清学调查。

导言：布鲁氏菌病仍然是对公共卫生的重大新威胁，因为它可以感染人类、野生动物、家畜和牲畜。因此，本研究旨在确定犬布鲁氏菌病（CB）的发病率，其与犬舍和农场犬的临床表现和生殖障碍的关系，以及其对公共卫生的重要性。材料与方法：于2022年1月至2023年12月，在伊朗克尔曼市育种场采集100只成年犬和50只牧羊犬共150份血液样本。采用Rose Bengal试验（RBT）和Wright试验对流产布鲁氏菌（B. abortus）和melitensis布鲁氏菌（B. melitensis）进行初步筛选，采用基于is711的常规PCR和实时聚合酶链反应（RT-PCR）对所有样本的布鲁氏菌属和种进行鉴定。结果：34%（34/100）犬有繁殖障碍史，30%（30/100）犬rbt阳性。此外，在犬科犬中还检出犬双球菌和abortus双球菌。16%（8/50）农场犬有繁殖障碍史，28%（14/50）为RBT阳性。此外，在农场犬中还检测到犬B. canis、abortus和melitensis。饲养未经巴氏消毒的牛奶与犬类RBT阳性显著相关（p=0.009），饲养障碍与犬类（p=0.045）和农场犬（p=0.03）血清阳性显著相关。结论：本研究首次检测到犬B. canis，表明对伊朗CB的认识取得了重大进展，揭示了犬B. melitensis和B. abortus是犬和农场犬的重要病原体，突出了该疾病在伊朗的公共卫生意义。

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来源期刊

Acta tropica 医学-寄生虫学

CiteScore

5.40

自引率

11.10%

发文量

383

审稿时长

37 days

期刊介绍： Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.