Super-resolution optical fluctuation imaging

IF 32.3 1区物理与天体物理 Q1 OPTICS

Nature Photonics Pub Date : 2025-01-10 DOI:10.1038/s41566-024-01571-3

Samrat Basak, Alexey Chizhik, José Ignacio Gallea, Ivan Gligonov, Ingo Gregor, Oleksii Nevskyi, Niels Radmacher, Roman Tsukanov, Jörg Enderlein

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Abstract

We present a comprehensive review of super-resolution optical fluctuation imaging (SOFI), a robust technique that leverages temporal fluctuations in fluorescence intensity to achieve super-resolution imaging without the need for single-molecule localization. The Review starts with a historical overview of super-resolution microscopy techniques, and then focuses on SOFI’s core principle—the analysis of intensity fluctuations using cumulants to improve spatial resolution. The paper discusses technical challenges, such as photobleaching, blinking kinetics and pixel size limitations, as well as proposing solutions like Fourier upsampling and balanced SOFI to mitigate these issues. Additionally, we discuss potential advancements in the field, including the integration of SOFI with other super-resolution modalities like structured illumination microscopy and image scanning microscopy, and the application of SOFI in cryo-fluorescence microscopy and quantum emitter-based imaging. This paper aims to serve as an essential resource for researchers interested in utilizing SOFI for high-resolution imaging in diverse biological applications.

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来源期刊

Nature Photonics 物理-光学

CiteScore

54.20

自引率

1.70%

发文量

158

审稿时长

12 months

期刊介绍： Nature Photonics is a monthly journal dedicated to the scientific study and application of light, known as Photonics. It publishes top-quality, peer-reviewed research across all areas of light generation, manipulation, and detection. The journal encompasses research into the fundamental properties of light and its interactions with matter, as well as the latest developments in optoelectronic devices and emerging photonics applications. Topics covered include lasers, LEDs, imaging, detectors, optoelectronic devices, quantum optics, biophotonics, optical data storage, spectroscopy, fiber optics, solar energy, displays, terahertz technology, nonlinear optics, plasmonics, nanophotonics, and X-rays. In addition to research papers and review articles summarizing scientific findings in optoelectronics, Nature Photonics also features News and Views pieces and research highlights. It uniquely includes articles on the business aspects of the industry, such as technology commercialization and market analysis, offering a comprehensive perspective on the field.