Regulation of antigen presentation and interleukin 10 production in murine dendritic cells via the oxidative stimulation of cell membrane using a polycation-porphyrin-conjugate-immobilized cell culture dish

IF 9.4 1区医学 Q1 ENGINEERING, BIOMEDICAL

Acta Biomaterialia Pub Date : 2025-02-01 DOI:10.1016/j.actbio.2025.01.004

Van Thi Hong Doan , Takashi Imai , Naoki Kawazoe , Guoping Chen , Toru Yoshitomi

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引用次数: 0

Abstract

Tolerogenic dendritic cells with professional antigen presentation via major histocompatibility complex molecules, co-stimulatory molecules (CD80/86), and interleukin 10 production have attracted significant attention as cellular therapies for autoimmune, allergic, and graft-versus-host diseases. In this study, we developed a cell culture dish equipped with polycation-porphyrin-conjugate-immobilized glass (PA-HP-G) to stimulate immature murine dendritic cell (iDCs). Upon irradiation with a red light at 635 nm toward the PA-HP-G surface, singlet oxygen was generated by the immobilized porphyrins on the PA-HP-G surface. When iDCs were cultured on the PA-HP-G surface, moderate light irradiation generated lipid radicals without excessive generation of reactive oxygen species in the cytoplasm and nucleus, which led to the oxidative stimulation of the iDC cell membrane without cell death. Light irradiation changed the morphology of dendritic cells on the PA-HP-G surface to a tree-like structure with dendrites, accelerated their maturation, and enhanced the antigen-presenting ability for the ovalbumin peptide via major histocompatibility complex class I molecules. Additionally, the antigen-presenting dendritic cells on the PA-HP-G surface produced the anti-inflammatory cytokine interleukin 10 upon light irradiation. These results indicated that upon moderate light irradiation, the PA-HP-G surface regulated the maturation of iDCs into tolerogenic dendritic cells.

Statement of Significance

• Cell culture dish is developed for selective oxidative stimulus of cell membrane.

• ¹O₂ is generated from polycation/porphyrin-immobilized glass by light irradiation.

• Lipid radicals are generated without generation of ROS in cytoplasm and nuclei.

• Immature dendritic cells are maturated by oxidative stimulation of cell membrane.

• Oxidative membrane stimulus enhances antigen-presentation and IL-10 secretion.

Abstract Image

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利用聚阳离子-卟啉偶联物-固定化细胞培养皿通过氧化刺激细胞膜调节树突状细胞抗原呈递和白细胞介素10的产生。

耐受性树突状细胞通过主要组织相容性复合体分子、共刺激分子（CD80/86）和白细胞介素10的产生来提供专业抗原，作为自身免疫性、过敏性和移植物抗宿主病的细胞疗法引起了极大的关注。在本研究中，我们研制了一种装有聚阳离子-卟啉偶联-固定化玻璃（PA-HP-G）的细胞培养皿来刺激未成熟小鼠树突状细胞（iDCs）。635 nm红光照射PA-HP-G表面后，固定化卟啉在PA-HP-G表面生成单线态氧。在PA-HP-G表面培养iDC时，适度光照产生脂质自由基，但细胞质和细胞核中不产生过多活性氧，导致iDC细胞膜氧化刺激，但细胞不死亡。光照使PA-HP-G表面的树突状细胞形态变为树突状结构，加速其成熟，并通过主要组织相容性复合体I类分子增强卵清蛋白肽的抗原呈递能力。此外，PA-HP-G表面的抗原呈递树突状细胞在光照射下产生抗炎细胞因子白细胞介素10。这些结果表明，在中等光照下，PA-HP-G表面调节了dc向耐受性树突状细胞的成熟。意义说明：•细胞培养皿用于选择性氧化刺激细胞膜。•光照射由多阳离子/卟啉固定玻璃产生1O2。•脂质自由基是在细胞质和细胞核中不产生ROS的情况下产生的。•未成熟的树突状细胞通过细胞膜的氧化刺激而成熟。•氧化膜刺激增强抗原呈递和IL-10分泌。

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来源期刊

Acta Biomaterialia 工程技术-材料科学：生物材料

CiteScore

16.80

自引率

3.10%

发文量

776

审稿时长

30 days

期刊介绍： Acta Biomaterialia is a monthly peer-reviewed scientific journal published by Elsevier. The journal was established in January 2005. The editor-in-chief is W.R. Wagner (University of Pittsburgh). The journal covers research in biomaterials science, including the interrelationship of biomaterial structure and function from macroscale to nanoscale. Topical coverage includes biomedical and biocompatible materials.