Diversity and composition of ocular microbiota in contact lens wearers: Efficacy of liposomal ozonated oil.

IF 4.1 3区医学 Q1 OPHTHALMOLOGY

Contact Lens & Anterior Eye Pub Date : 2025-01-08 DOI:10.1016/j.clae.2025.102368

María Carmen Sánchez-González, Inmaculada Gallardo-Real, Estanislao Gutiérrez-Sánchez, Concepción De-Hita-Cantalejo, Raúl Capote-Puente, José-María Sánchez-González

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引用次数: 0

Abstract

Purpose: To characterize the ocular surface microbiota in regular contact lens wearers with dry eyes and assess the effectiveness of reducing bacterial load using a liposomal ozonated oil solution.

Methods: This prospective, longitudinal, controlled study randomized subjects into two groups. Group A (45 subjects) received hydroxypropylmethylcellulose (HPMC, Artific®), while Group B (41 subjects) received ozonated sunflower seed oil with soybean phospholipids (OSSO, Ozonest®). Microbial communities were analyzed via DNA metabarcoding of the 16S rRNA gene, and statistical analyses (alpha and beta diversity) were performed in R.

Results: Both groups predominantly harbored Staphylococcus caprae, Streptococcus oralis, and Corynebacterium spp., with OSSO and HPMC users showing distinct bacterial profiles. Alpha diversity showed no significant differences, but beta diversity revealed differences in bacterial composition between the groups.

Conclusions: The results seem to indicate that the use of ozonized oil reduces the bacterial load compared to the solution used as a control.

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隐形眼镜佩戴者眼部微生物群的多样性和组成：臭氧化脂质体油的功效。

目的：研究干眼症常规隐形眼镜佩戴者眼表微生物群特征，并评估使用臭氧化脂质体油溶液降低细菌负荷的有效性。方法：本前瞻性、纵向、对照研究将受试者随机分为两组。A组（45例）接受羟丙基甲基纤维素（HPMC, Artific®）治疗，B组（41例）接受大豆磷脂（OSSO, Ozonest®）臭氧化葵花籽油治疗。通过16S rRNA基因的DNA元条形码分析了微生物群落，并对r进行了统计分析（α和β多样性）。结果：两组主要含有卡葡萄球菌、口腔链球菌和棒状杆菌，OSSO和HPMC用户显示出不同的细菌谱。α多样性无显著差异，但β多样性揭示了不同组间细菌组成的差异。结论：结果似乎表明，使用臭氧化油减少细菌负荷相比，作为对照的溶液使用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Contact Lens & Anterior Eye OPHTHALMOLOGY-

CiteScore

7.60

自引率

18.80%

发文量

198

审稿时长

55 days

期刊介绍： Contact Lens & Anterior Eye is a research-based journal covering all aspects of contact lens theory and practice, including original articles on invention and innovations, as well as the regular features of: Case Reports; Literary Reviews; Editorials; Instrumentation and Techniques and Dates of Professional Meetings.