Resistance of Populus davidiana × P. bolleana overexpressing cinnamoyl-CoA reductase gene to Lymantria dispar larvae.

IF 2.7 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Transgenic Research Pub Date : 2025-01-09 DOI:10.1007/s11248-024-00426-5

Ye Li, Ruiqiong Zhang, Lili Sun, Chuanwang Cao

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Abstract

Lignin is a crucial defense phytochemical against phytophagous insects. Cinnamoyl-CoA reductase (CCR) is a key enzyme in lignin biosynthesis. In this study, transgenic Populus davidiana × P. bolleana overexpressing the PdbCCR gene were generated via Agrobacterium-mediated transformation. Successful integration of PdbCCR into the poplar genome was confirmed by PCR amplification and quantitative reverse transcription PCR (qRT-PCR). The lignin content in the transgenic poplar leaves was significantly higher than that in the wild poplar, and after L. dispar larvae fed on the transgenic poplar, the CCR activity was clearly induced. The L. dispar larvae grew slowly after feeding on transgenic poplar and the laccase, cellulase and three detoxifying enzymes were induced compared with larvae after feeding on wild-type poplar. The bioassay further revealed that transgenic poplar plants overexpressing PdbCCR showed a high level of resistance to L. dispar larvae. These results confirmed that PdbCCR is a candidate gene for breeding insect resistant poplar.

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山杨的抗性研究。过表达肉桂酰辅酶a还原酶基因对异毒蛾幼虫的影响。

木质素是一种重要的防御植物性化学物质。肉桂酰辅酶a还原酶（CCR）是木质素生物合成的关键酶。在本研究中，转基因大叶杨× P。通过农杆菌介导的转化产生过表达PdbCCR基因的bolleana。通过PCR扩增和定量反转录PCR （qRT-PCR）证实PdbCCR成功整合到杨树基因组中。转基因杨树叶片中木质素含量显著高于野生杨树，且异斑夜蛾幼虫取食转基因杨树后，CCR活性明显被诱导。以转基因杨树为食后，异色夜蛾幼虫生长缓慢，其漆酶、纤维素酶和3种解毒酶均较野生型杨树为食后有所提高。生物实验进一步表明，过表达PdbCCR的转基因杨树对异斑叶枯病幼虫表现出较高的抗性。这些结果证实PdbCCR是杨树抗虫育种的候选基因。

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来源期刊

Transgenic Research 生物-生化研究方法

CiteScore

5.40

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Transgenic Research focusses on transgenic and genome edited higher organisms. Manuscripts emphasizing biotechnological applications are strongly encouraged. Intellectual property, ethical issues, societal impact and regulatory aspects also fall within the scope of the journal. Transgenic Research aims to bridge the gap between fundamental and applied science in molecular biology and biotechnology for the plant and animal academic and associated industry communities. Transgenic Research publishes -Original Papers -Reviews: Should critically summarize the current state-of-the-art of the subject in a dispassionate way. Authors are requested to contact a Board Member before submission. Reviews should not be descriptive; rather they should present the most up-to-date information on the subject in a dispassionate and critical way. Perspective Reviews which can address new or controversial aspects are encouraged. -Brief Communications: Should report significant developments in methodology and experimental transgenic higher organisms