Identification of genetic loci and candidate genes underlying Fusarium crown rot resistance in wheat.

IF 4.4 1区农林科学 Q1 AGRONOMY

Theoretical and Applied Genetics Pub Date : 2025-01-08 DOI:10.1007/s00122-025-04818-x

Yuqing Su, Xiangru Xu, Yunqiao Wang, Tongzhu Wang, Jiazheng Yu, Jiatian Yang, Jinlong Li, Yutian Gao, Yixin Wang, Wei Sang, Cong Li, Xingyi Wang, Zhi Zheng, Chaojie Xie, Jian Ma, Jun Ma

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引用次数: 0

Abstract

Key message: A major locus Qfcr.cau-1B conferring resistance to Fusarium crown rot was identified and validated. The putative gene underlying this locus was pinpointed via virus-induced gene silencing. Fusarium crown rot (FCR), caused by various Fusarium pathogens such as Fusarium pseudograminearum and F. culmorum, is a severe soil-borne disease which significantly affected wheat (Triticum aestivum) production in many arid and semi-arid cropping regions of the world. In this study, a total of 5 QTLs associated with FCR resistance were detected on chromosomes 1B, 2B, 3A, 5A, and 7D using a population of 120 F₈ recombinant inbred lines (RIL) derived from a cross between two Chinese germplasm 20828 and SY95-71. A major locus Qfcr.cau-1B, which accounted for up to 28.33% of the phenotypic variation with a LOD value of 10.99, was consistently detected across all three trials conducted. The effect of Qfcr.cau-1B on FCR resistance was further validated using a F₅ RIL population between 20828 and BLS2. Integrated transcriptome and sequence variation analysis showed that three genes including TraesCS1B02G017700, TraesCS1B02G016400, and TraesCS1B02G022300 were potential candidate genes for Qfcr.cau-1B. Of these three genes, the virus-induced silencing of TraesCS1B02G022300 significantly promoted FCR severity, indicating its positive role in FCR resistance. Taken together, results from this study expand our understanding on genetic basis of FCR resistance in wheat and will be indicative for cloning genes conferring FCR resistance.

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小麦抗枯萎病冠腐病遗传位点及候选基因的鉴定。

关键信息：主要位点Qfcr。鉴定并验证了对镰刀菌冠腐病具有抗性的cac - 1b。该基因座的假定基因是通过病毒诱导的基因沉默确定的。镰刀菌冠腐病（Fusarium crown rot， FCR）是一种严重的土传病害，由多种镰刀菌（Fusarium pseudograminearum）和镰刀菌（F. culmorum）等病原菌引起，严重影响了世界上许多干旱和半干旱种植区的小麦生产。本研究以中国种质资源20828与SY95-71杂交的120个F8重组自交系（RIL）为材料，在1B、2B、3A、5A和7D染色体上共检测到5个与FCR抗性相关的qtl。一个主要的轨迹Qfcr。cac - 1b占表型变异的28.33%，LOD值为10.99，在所有三项试验中均被一致检测到。Qfcr的效果。在20828和BLS2之间的F5 RIL群体中进一步验证了cac - 1b对FCR耐药的作用。整合转录组和序列变异分析显示，TraesCS1B02G017700、TraesCS1B02G016400和TraesCS1B02G022300是Qfcr.cau-1B的潜在候选基因。在这三个基因中，病毒诱导的TraesCS1B02G022300的沉默显著提高了FCR的严重程度，表明其在FCR抗性中具有积极作用。综上所述，本研究结果扩大了我们对小麦FCR抗性遗传基础的认识，并将为克隆FCR抗性基因提供指导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Theoretical and Applied Genetics 生物-农艺学

CiteScore

9.60

自引率

7.40%

发文量

241

审稿时长

2.3 months

期刊介绍： Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.