Relationship Between HSV-1 Serostatus and HSV-2 IgG Confirmation Results Using an Inhibition Assay.

IF 2.4 4区 医学 Q3 INFECTIOUS DISEASES
Tessa M Shates, Susan Realegeno, Harry E Prince, Elizabeth M Marlowe
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引用次数: 0

Abstract

Background: Approximately 2% of HerpeSelect herpes simples virus type 2 (HSV-2) IgG enzyme immunoassay (screen assay) sera-positive samples do not confirm using an HSV-2 IgG inhibition assay. Of these, roughly 1.33% are confirmed negative, and a small proportion (0.2%) are indeterminate due to inhibition observed with HSV-1 and HSV-2 lysate. The objective of this study was to assess the relationship between HSV-1 IgG serostatus and HSV-2 IgG confirmation results using the HSV-2 IgG inhibition assay.

Methods: This study included consecutive HSV-2 IgG screen-positive specimens regardless of index value. Specimens were also tested for HSV-1 IgG. The HSV-2 IgG confirmation results from the inhibition assay were evaluated to assess the relationship between inhibition results and HSV-1 serostatus.

Results: Of the 21,006 positive HSV-2 IgG specimens, 13,237 (63%) were also positive for HSV-1 IgG. The proportion of HSV-1 IgG-positive samples among inhibition-indeterminate samples (33 to 40 [83%]) was higher than among inhibition-negative (167 of 279 [60%]) and inhibition-positive (13,041 of 20,691 [63%]) samples. Among HSV-1 IgG-positive samples, the median HSV-1 IgG index was also significantly higher for the inhibition-indeterminate group (median, 48.8) than for the inhibition-negative (median, 30.7) and inhibition-positive groups (median, 31.2; P = 0.00013 and P = 3.1e - 05, respectively).

Conclusion: These findings suggest that HSV-2 IgG inhibition-indeterminate, but not inhibition-negative, results are associated with higher index values of HSV-1 IgG. Further studies are needed to understand other factors associated with inhibition-indeterminate results.

抑制试验检测HSV-1血清状态与HSV-2 IgG确认结果的关系
背景:大约2%的疱疹选择性单纯疱疹病毒2型(HSV-2) IgG酶免疫测定(筛选试验)血清阳性样本不证实使用HSV-2 IgG抑制试验。其中,约1.33%被证实为阴性,小部分(0.2%)由于被HSV-1和HSV-2裂解物抑制而不确定。本研究的目的是通过HSV-2 IgG抑制试验评估HSV-1 IgG血清状态与HSV-2 IgG确认结果的关系。方法:本研究包括连续的HSV-2 IgG筛查阳性标本,不论指标值。同时对标本进行HSV-1 IgG检测。对抑制试验的HSV-2 IgG确认结果进行评估,以评估抑制结果与HSV-1血清状态的关系。结果:21006份HSV-2 IgG阳性标本中,13237份(63%)HSV-1 IgG阳性。在抑制不确定样本中,HSV-1 igg阳性样本的比例(33 ~ 40份[83%])高于抑制阴性样本(279份中有167份[60%])和抑制阳性样本(20691份中有13041份[63%])。在HSV-1 IgG阳性样本中,抑制不确定组的HSV-1 IgG指数中位数(48.8)也显著高于抑制阴性组(30.7)和抑制阳性组(31.2;P = 0.00013和P = 3.1e - 05)。结论:HSV-2 IgG抑制不确定,但非抑制阴性,结果与较高的HSV-1 IgG指标值相关。需要进一步的研究来了解与抑制相关的其他因素-不确定的结果。
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来源期刊
Sexually transmitted diseases
Sexually transmitted diseases 医学-传染病学
CiteScore
4.00
自引率
16.10%
发文量
289
审稿时长
3-8 weeks
期刊介绍: ​Sexually Transmitted Diseases, the official journal of the American Sexually Transmitted Diseases Association​, publishes peer-reviewed, original articles on clinical, laboratory, immunologic, epidemiologic, behavioral, public health, and historical topics pertaining to sexually transmitted diseases and related fields. Reports from the CDC and NIH provide up-to-the-minute information. A highly respected editorial board is composed of prominent scientists who are leaders in this rapidly changing field. Included in each issue are studies and developments from around the world.
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