BBX22 enhances the accumulation of antioxidants to inhibit DNA damage and promotes DNA repair under high UV-B.

Abstract

Under changing climatic conditions, plant exposure to high-intensity UV-B can be a potential threat to plant health and all plant-derived human requirements, including food. It's crucial to understand how plants respond to high UV-B radiation so that proper measures can be taken to enhance tolerance towards high UV-B stress. We found that BBX22, a B-box protein-coding gene, is strongly induced within one hour of exposure to high-intensity UV-B. Our metabolomics data indicated that BBX22 promotes the accumulation of antioxidants like ascorbic acid and proline. These antioxidants play a vital role in shielding plants exposed to high UV-B from the detrimental effects of Reactive Oxygen Species (ROS), including DNA damage. Additionally, BBX22 promotes DNA damage repair by inducing the expression of DNA repair genes like UVR1 and UVR3. BBX22 directly binds to the promoter of UVR1 to regulate its expression. Furthermore, BBX22 indirectly induces the expression of UVR1 and UVR3 by enhancing the binding of HY5 to their promoters. Together, these results suggest a multi-pronged role of BBX22 in protection against high-intensity UV-B. Enhancing BBX22 levels or its orthologs in different plant species can potentially offer DNA damage protection and tolerance against intense UV radiation.