Impact of trace metal supplementation on anaerobic biological methanation under hydrogen and carbon dioxide starvation.

IF 7.8 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

npj Biofilms and Microbiomes Pub Date : 2025-01-08 DOI:10.1038/s41522-025-00649-2

G Ghiotto, N De Bernardini, E Orellana, G Fiorito, L Cenci, P G Kougias, S Campanaro, L Treu

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Abstract

Biomethanation is a crucial process occurring in natural and engineered systems which can reduce carbon dioxide to methane impacting the global carbon cycle. However, little is known about the effect of on-and-off gaseous provision and micronutrients on bioconversion. Here, anaerobic microbiomes underwent intermittent feeding with incremental starvations and selective metal supplementation to assess the impact of hydrogen and carbon dioxide availability on microbial physiology. Resilience was tested under differential cultivations in basal medium supplemented with either nickel or cobalt. Nickel-augmented cultures exhibited faster recovery upon starvation, suggesting a beneficial effect. Dominant Methanothermobacter thermautotrophicus demonstrated robust growth, genetic stability and transcriptional downregulation when starved. Conversely, bacteria were plastic and prone to genetic fluctuations, accumulating mutations on genes encoding for ABC-transporters and C-metabolism enzymes. This study pioneers cellular resilience and response to micronutrient supplementation in anaerobic carbon dioxide-fixating microbiomes, offering valuable insights into microbial activity recovery after carbon and electron donor deprivation.

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补充微量金属对氢和二氧化碳饥饿条件下厌氧生物甲烷化的影响。

生物甲烷化是发生在自然和工程系统中的一个关键过程，它可以将二氧化碳转化为甲烷，影响全球碳循环。然而，关于开关气体供应和微量营养素对生物转化的影响知之甚少。在这里，厌氧微生物组通过间歇性喂养，增加饥饿和选择性补充金属来评估氢和二氧化碳对微生物生理的影响。在分别添加镍或钴的基础培养基上进行差异培养试验。镍增强培养物在饥饿时恢复得更快，表明其有益效果。优势产热自养甲烷菌在饥饿状态下表现出强劲的生长、遗传稳定性和转录下调。相反，细菌具有可塑性，易于遗传波动，在编码abc转运蛋白和c代谢酶的基因上积累突变。这项研究是厌氧二氧化碳固定微生物群中细胞恢复能力和对微量营养素补充的反应的先驱，为碳和电子供体剥夺后微生物活性恢复提供了有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology

CiteScore

12.10

自引率

3.30%

发文量

审稿时长

9 weeks

期刊介绍： npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.