Integrative Analysis of the Role of MRPL21 in Human Pan-Cancer and Its Relationship with the Progression of Lung Adenocarcinoma.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biotechnology Pub Date : 2025-01-07 DOI:10.1007/s12033-024-01348-7

Qi Xu, Jiale Wang, Jing Wang, Ou Zhang, Yuwan Gao, Xiaoqiao Cui, Chengyi Zhao, Feng Liu, Xiaohui Chen

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引用次数: 0

Abstract

Mitochondrial ribosomal protein L21 (MRPL21) is essential for normal cell function and may play a significant role in cancer development. In this study, we performed a comprehensive pan-cancer analysis to explore MRPL21's function across different cancers, utilizing multiple online data platforms such as TCGA. Our analysis covered its clinical significance and biological functions, including expression levels, survival and diagnostic analysis, gene mutations, multidimensional immune-correlation analysis, tumor heterogeneity, and cancer-associated signaling pathways. Additionally, we constructed a prognostic nomogram for lung adenocarcinoma (LUAD) patients based on MRPL21 and validated its biological function through in vitro experiments. Our findings revealed that MRPL21 is commonly overexpressed in various cancers and is associated with poor prognosis. It significantly impacts cancer-related pathways, particularly those related to cell cycle activation. Moreover, MRPL21 is critical in the tumor microenvironment and is closely linked to immune infiltration across several cancer types. Its expression correlates with essential factors such as tumor mutational burden, microsatellite instability, immune checkpoint, and methylation patterns. In LUAD, MRPL21 was identified as an independent risk factor and demonstrated that MRPL21 promotes LUAD progression. Overall, MRPL21 holds potential as both a diagnostic and prognostic marker in cancer and could serve as a promising therapeutic target, particularly for LUAD.

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MRPL21在人类泛癌中的作用及其与肺腺癌进展关系的综合分析。

线粒体核糖体蛋白L21 （MRPL21）对正常细胞功能至关重要，并可能在癌症发展中发挥重要作用。在本研究中，我们利用多个在线数据平台（如TCGA）进行了全面的泛癌症分析，以探索MRPL21在不同癌症中的功能。我们的分析涵盖了其临床意义和生物学功能，包括表达水平、生存和诊断分析、基因突变、多维免疫相关分析、肿瘤异质性和癌症相关信号通路。此外，我们基于MRPL21构建了肺腺癌（LUAD）患者的预后图，并通过体外实验验证了其生物学功能。我们的研究结果表明，MRPL21在各种癌症中普遍过表达，并与不良预后相关。它显著影响癌症相关途径，特别是与细胞周期激活相关的途径。此外，MRPL21在肿瘤微环境中至关重要，并与多种癌症类型的免疫浸润密切相关。其表达与肿瘤突变负担、微卫星不稳定性、免疫检查点和甲基化模式等重要因素相关。在LUAD中，MRPL21被确定为一个独立的危险因素，并证明MRPL21促进LUAD的进展。总的来说，MRPL21具有作为癌症诊断和预后标志物的潜力，可以作为一个有希望的治疗靶点，特别是对于LUAD。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.