UV-C Exposure Enhanced the Cd2+ Adsorption Capability of the Radiation-Resistant Strain Sphingomonas sp. M1-B02.

IF 4.1 2区 生物学 Q2 MICROBIOLOGY
Yunshi Li, Haoyuan Niu, Shuang Li, Ming Yue, Gaosen Zhang
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Abstract

Microbial adsorption is a cost-effective and environmentally friendly remediation method for heavy metal pollution. The adsorption mechanism of cadmium (Cd) by bacteria inhabiting extreme environments is largely unexplored. This study describes the biosorption of Cd2+ by Sphingomonas sp. M1-B02, which was isolated from the moraine on the north slope of Mount Everest and has a good potential for biosorption. The difference in Cd2+ adsorption of the strain after UV irradiation stimulation indicated that the adsorption reached 68.90% in 24 h, but the adsorption after UV irradiation increased to 80.56%. The genome of strain M1-B02 contained antioxidant genes such as mutL, recA, recO, and heavy metal repair genes such as RS14805, apaG, chrA. Hydroxyl, nitro, and etceteras bonds on the bacterial surface were involved in Cd2+ adsorption through complexation reactions. The metabolites of the strains were significantly different after 24 h of Cd2+ stress, with pyocyanin, L-proline, hypoxanthine, etc., being downregulated and presumably involved in Cd2+ biosorption and upregulated after UV-C irradiation, which may explain the increase in Cd2+ adsorption capacity of the strain after UV-C irradiation, while the strain improved the metabolism of the antioxidant metabolite carnosine, indirectly increasing the adsorption capacity of the strains for Cd2+.

UV-C暴露增强抗辐射菌株鞘氨单胞菌M1-B02对Cd2+的吸附能力。
微生物吸附是一种经济、环保的重金属污染修复方法。极端环境下细菌对镉的吸附机理目前还未得到充分的研究。本文研究了从珠穆朗玛峰北坡冰碛中分离得到的具有良好生物吸附潜力的鞘单胞菌M1-B02对Cd2+的生物吸附作用。紫外照射后菌株对Cd2+的吸附差异表明,24 h吸附量达到68.90%,而紫外照射后的吸附量增加到80.56%。菌株M1-B02基因组中含有mutL、recA、recO等抗氧化基因和RS14805、apaG、chrA等重金属修复基因。细菌表面的羟基、硝基等键通过络合反应参与了Cd2+的吸附。菌株的代谢产物在Cd2+胁迫24 h后差异显著,pyocyanin、l -脯氨酸、次黄嘌呤等被下调,可能参与了Cd2+的生物吸附,而在UV-C照射后则上调,这可能解释了菌株在UV-C照射后Cd2+吸附能力增加的原因,同时菌株改善了抗氧化代谢物肌肽的代谢,间接增加了菌株对Cd2+的吸附能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microorganisms
Microorganisms Medicine-Microbiology (medical)
CiteScore
7.40
自引率
6.70%
发文量
2168
审稿时长
20.03 days
期刊介绍: Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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