Novel Betaherpesviruses in Neotropical Bats on the Caribbean Island of St. Kitts: First Report from Antillean Tree Bats (Ardops nichollsi) and Evidence for Cross-Species Transmission.

IF 4.1 2区 生物学 Q2 MICROBIOLOGY
Jessica L Kulberg, Sarah Hooper, Yashpal S Malik, Souvik Ghosh
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Abstract

To date, limited information is available on herpesviruses in bats from the Caribbean region. We report here high detection rates (24.24%, n = 66) of herpesviruses in oral samples from apparently healthy bats (Ardops nichollsi (75%, 9/12) and Molossus molossus (28%, 7/25)) on the Lesser Antillean Island of St. Kitts. Based on analysis of partial DNA polymerase (DPOL) sequences (~225 amino acid (aa) residues), we identified two distinct groups of herpesviruses (BO-I and -II) that were unique to A. nichollsi and M. molossus, respectively. Within the subfamily Betaherpesvirinae, the BO-I DPOL sequences shared low deduced aa identities (<70%) with other herpesviruses, and phylogenetically, they formed a distinct cluster, representing a putative novel betaherpesvirus. The BO-II DPOL sequences were closely related to a putative novel betaherpesvirus from a M. molossus in Lesser Antillean Island of Martinique, indicating possible transmission of herpesviruses by bat movement between the Caribbean Islands. Phylogenetically, the BO-I and -II betaherpesviruses exhibited species-specific (A. nichollsi and M. molossus, respectively) as well as family-specific (Phyllostomidae and Molossidae, respectively) clustering patterns, corroborating the hypothesis on host specificity of betaherpesviruses. Interestingly, a single M. molossus betaherpesvirus strain clustered with the A. nichollsi betaherpesviruses, indicating possible interspecies transmission of herpesviruses between Phyllostomidae and Molossidae. To our knowledge, this is the first report on detection of herpesviruses from Antillean tree bats (A. nichollsi), expanding the host range of betaherpesviruses. Taken together, the present study identified putative novel betaherpesviruses that might be unique to chiropteran species (A. nichollsi and M. molossus), indicating virus-host coevolution, and provided evidence for interspecies transmission of betaherpesviruses between chiropteran families.

加勒比圣基茨岛新热带蝙蝠中的新型乙型疱疹病毒:来自安的列斯树蝠(Ardops nichollsi)的首次报告和跨物种传播的证据。
迄今为止,关于加勒比地区蝙蝠疱疹病毒的信息有限。我们报告了在圣基茨小安的列斯岛表面健康的蝙蝠(阿多普斯尼科尔西(75%,9/12)和Molossus Molossus(28%, 7/25))的口腔样本中疱疹病毒的高检出率(24.24%,n = 66)。根据部分DNA聚合酶(DPOL)序列(约225个氨基酸(aa)残基)的分析,我们鉴定出了两个不同的疱疹病毒群(bo - 1和-II),它们分别是A. nichollsi和M. molossus所特有的。在乙型疱疹病毒亚科中,bo - 1 DPOL序列与马提尼克岛小安的列斯岛的M. molossus具有低推定的aa特征,表明可能通过蝙蝠在加勒比群岛之间的运动传播疱疹病毒。从系统发育上看,bo - 1型和β - 2型乙型疱疹病毒表现出物种特异性(分别为A. nichollsi和M. molossus)和家族特异性(分别为Phyllostomidae和Molossidae)的聚类模式,证实了β -疱疹病毒宿主特异性的假设。有趣的是,一个单独的molossus betaherpesvirus株与a . nichollsi betaherpesvirus聚集在一起,表明疱疹病毒可能在phyllostomides科和Molossidae科之间进行种间传播。据我们所知,这是在安的列斯树蝠(A. nichollsi)中检测到疱疹病毒的第一份报告,扩大了乙型疱疹病毒的宿主范围。综上所述,本研究确定了可能为翼类动物(A. nichollsi和M. molossus)所特有的新型β -疱疹病毒,表明病毒与宿主共同进化,并为翼类动物科之间的β -疱疹病毒种间传播提供了证据。
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来源期刊
Microorganisms
Microorganisms Medicine-Microbiology (medical)
CiteScore
7.40
自引率
6.70%
发文量
2168
审稿时长
20.03 days
期刊介绍: Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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