Newcastle Disease Virus-Vectored African Swine Fever Virus Antigen Cocktail Delays the Onset of ASFV-SY18 but Is Not Protective.

IF 4.1 2区 生物学 Q2 MICROBIOLOGY
Qian Li, Yiqian Jiang, Min Zheng, Xuefei Sun, Lili Hui, Yanyan Zhang, Huixian Yue, Yu Qi, Siqi Li, Junnan Ke, Qixuan Li, Boli Ma, Xiaoying Jia, Fengjie Wang, Lijuan Mi, Shoufeng Zhang, Faming Miao, Shuchao Wang, Fei Zhang, Teng Chen, Rongliang Hu
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引用次数: 0

Abstract

African Swine Fever (ASF) is a highly contagious viral disease threatening the global pig industry. Currently, only two gene-deleted live attenuated vaccines are approved, exclusively in Vietnam, and their long-term effectiveness and safety are unproven, prompting the need for safer alternatives. This study assessed a cocktail of African Swine Fever Virus (ASFV) antigens delivered via a recombinant Newcastle Disease Virus (rNDV) vector against the genotype II ASFV-SY18. Antigens pB602L, pEP84R, and p22 (pKP177R) were selected based on virus neutralization and lymphocyte proliferation assays in mice and combined with capsid protein p72 (pB646L) for vaccination and challenge in pigs. The antigen cocktail delayed ASF symptoms by 3-4 days but did not prevent the lethal ASFV-SY18 infection. Significant ASFV-specific gamma interferon (IFN-γ) positive responses and NDV antibodies were detected post-inoculation, showing an induced immune response, though ASFV-specific p72 antibodies were absent. The cocktail did not cause cytokine imbalance, indicating the vector's safety in pigs. Despite some delay in disease progression, the protection against genotype II ASFV was inadequate, underscoring the need to select more effective antigens and enhance immune responses for virus-vectored vaccines.

新城疫病毒载体非洲猪瘟病毒抗原混合物延迟ASFV-SY18的发病,但没有保护作用。
非洲猪瘟(ASF)是一种威胁全球养猪业的高度传染性病毒性疾病。目前,只有两种基因缺失减毒活疫苗获得批准,而且只在越南获得批准,而且它们的长期有效性和安全性尚未得到证实,这促使人们需要更安全的替代品。本研究评估了通过重组新城疫病毒(rNDV)载体传递的非洲猪瘟病毒(ASFV)抗原混合物对抗II型ASFV- sy18的效果。根据小鼠病毒中和和淋巴细胞增殖试验选择抗原pB602L、pEP84R和p22 (pKP177R),并与衣壳蛋白p72 (pB646L)联合接种和攻毒猪。抗原鸡尾酒使ASF症状延迟3-4天,但不能预防致命的ASFV-SY18感染。接种后检测到显著的asfv特异性γ干扰素(IFN-γ)阳性反应和NDV抗体,显示诱导免疫应答,尽管asfv特异性p72抗体缺失。混合物未引起细胞因子失衡,表明载体在猪体内是安全的。尽管疾病进展有所延迟,但对基因型ASFV的保护是不够的,这强调了选择更有效的抗原和增强病毒载体疫苗免疫反应的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microorganisms
Microorganisms Medicine-Microbiology (medical)
CiteScore
7.40
自引率
6.70%
发文量
2168
审稿时长
20.03 days
期刊介绍: Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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