Characterization and Molecular Insights of a Chromium-Reducing Bacterium Bacillus tropicus.

IF 4.1 2区 生物学 Q2 MICROBIOLOGY
Shanjana Rahman Tuli, Md Firoz Ali, Tabassum Binte Jamal, Md Abu Sayem Khan, Nigar Fatima, Irfan Ahmed, Masuma Khatun, Shamima Akhtar Sharmin
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Abstract

Environmental pollution from metal toxicity is a widespread concern. Certain bacteria hold promise for bioremediation via the conversion of toxic chromium compounds into less harmful forms, promoting environmental cleanup. In this study, we report the isolation and detailed characterization of a highly chromium-tolerant bacterium, Bacillus tropicus CRB14. The isolate is capable of growing on 5000 mg/L Cr (VI) in an LB (Luria Bertani) agar plate while on 900 mg/L Cr (VI) in LB broth. It shows an 86.57% reduction ability in 96 h of culture. It can also tolerate high levels of As, Cd, Co, Fe, Zn, and Pb. The isolate also shows plant growth-promoting potential as demonstrated by a significant activity of nitrogen fixation, phosphate solubilization, IAA (indole acetic acid), and siderophore production. Whole-genome sequencing revealed that the isolate lacks Cr resistance genes in their plasmids and are located on its chromosome. The presence of the chrA gene points towards Cr(VI) transport, while the absence of ycnD suggests alternative reduction pathways. The genome harbors features like genomic islands and CRISPR-Cas systems, potentially aiding adaptation and defense. Analysis suggests robust metabolic pathways, potentially involved in Cr detoxification. Notably, genes for siderophore and NRP-metallophore production were identified. Whole-genome sequencing data also provides the basis for molecular validation of various genes. Findings from this study highlight the potential application of Bacillus tropicus CRB14 for bioremediation while plant growth promotion can be utilized as an added benefit.

一种还原铬的热带芽孢杆菌的特性及分子研究。
金属毒性引起的环境污染是一个广泛关注的问题。某些细菌有望通过将有毒铬化合物转化为危害较小的形式来进行生物修复,从而促进环境清理。在这项研究中,我们报道了一种高度耐铬的细菌,热带芽孢杆菌CRB14的分离和详细的表征。该分离菌能在5000 mg/L Cr (VI)的LB (Luria Bertani)琼脂板上生长,在900 mg/L Cr (VI)的LB肉汤上生长。培养96 h,还原率达86.57%。它还能耐受高水平的砷、镉、钴、铁、锌和铅。该分离物还显示出促进植物生长的潜力,这表明其具有显著的固氮、磷酸盐增溶、IAA(吲哚乙酸)和铁载体生产活性。全基因组测序结果显示,该分离株在质粒中缺乏Cr抗性基因,并位于其染色体上。chrA基因的存在指向了Cr(VI)的转运,而ycnD的缺失提示了其他的还原途径。基因组具有基因组岛和CRISPR-Cas系统等特征,可能有助于适应和防御。分析表明强大的代谢途径,可能参与铬解毒。值得注意的是,鉴定出了铁载体和nrp -金属载体产生的基因。全基因组测序数据也为各种基因的分子验证提供了依据。本研究结果表明,在促进植物生长的同时,热带芽孢杆菌CRB14在生物修复方面具有潜在的应用潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microorganisms
Microorganisms Medicine-Microbiology (medical)
CiteScore
7.40
自引率
6.70%
发文量
2168
审稿时长
20.03 days
期刊介绍: Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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