Genomic and Phenotypic Characterization of Streptomyces sirii sp. nov., Amicetin-Producing Actinobacteria Isolated from Bamboo Rhizospheric Soil.

IF 4.1 2区 生物学 Q2 MICROBIOLOGY
Yuliya V Zakalyukina, Vera A Alferova, Arina A Nikandrova, Albina R Kiriy, Alisa P Chernyshova, Marsel R Kabilov, Olga A Baturina, Mikhail V Biryukov, Petr V Sergiev, Dmitrii A Lukianov
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引用次数: 0

Abstract

In our large-scale search for antimicrobial-producing bacteria, we isolated an actinomycete strain from rhizospheric soil of Bambusa vulgaris. The strain designated BP-8 showed noticeable antibacterial activity. BP-8 was subjected to a whole-genome analysis via a polyphasic taxonomy approach, and its antibacterial metabolite was identified by HRLS-MS. The results of the physiological and morphological analyses indicated that BP-8 is an aerobic, neutrophilic, mesophilic organism that is tolerant to 8% NaCl and can use a wide range of carbohydrates. It forms curly sporophores with a warty surface. The results of the phylogenetic and average nucleotide identity analyses and in silico DNA-DNA hybridization calculation indicated that BP-8 represents the type strain of a novel Streptomyces species. A comparative in silico analysis of the genome sequences of BP-8 and its closest related strains revealed the presence of genes encoding chemotaxonomic markers characteristic of Streptomyces. The antibacterial compound was identified as amicetin. Genomic mining also revealed more than 10 biosynthetic gene clusters that have not been described previously and may lead to the discovery of new valuable compounds. On the basis of these results, strain BP-8T (=VKM Ac-3066T = CCTCC AA 2024094T) is proposed as the type strain of the novel species Streptomyces sirii sp. nov.

竹根际土壤中产氨基糖苷链霉菌sirii sp. nov.放线菌的基因组和表型特征
在我们对抗菌细菌的大规模研究中,我们从普通竹根际土壤中分离出一株放线菌。菌株BP-8表现出明显的抗菌活性。采用多相分类学方法对BP-8进行全基因组分析,并通过HRLS-MS鉴定其抗菌代谢物。生理和形态分析结果表明,BP-8是一种耐8% NaCl的好氧、嗜中性、中温微生物,能利用多种碳水化合物。它形成卷曲的孢子体,表面有疣状。系统发育分析、平均核苷酸同源性分析和DNA-DNA杂交计算结果表明,BP-8为链霉菌新种的类型菌株。通过对BP-8及其近缘菌株基因组序列的比较分析,发现存在链霉菌特有的化学分类标记基因。抗菌化合物鉴定为氨苄菌素。基因组挖掘还揭示了10多个以前未被描述的生物合成基因簇,并可能导致发现新的有价值的化合物。在此基础上,提出菌株BP-8T (=VKM Ac-3066T = CCTCC AA 2024094T)为新种sirii Streptomyces sp. nov的型菌株。
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来源期刊
Microorganisms
Microorganisms Medicine-Microbiology (medical)
CiteScore
7.40
自引率
6.70%
发文量
2168
审稿时长
20.03 days
期刊介绍: Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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